Journal of the American Oil Chemists' Society

, Volume 79, Issue 2, pp 159–163

Conjugated linoleic acid production from linoleic acid by lactic acid bacteria

  • Shigenobu Kishino
  • Jun Ogawa
  • Yoriko Omura
  • Kenji Matsumura
  • Sakayu Shimizu
Article

DOI: 10.1007/s11746-002-0451-4

Cite this article as:
Kishino, S., Ogawa, J., Omura, Y. et al. J Amer Oil Chem Soc (2002) 79: 159. doi:10.1007/s11746-002-0451-4

Abstract

After screening 14 genera of lactic acid bacteria, Lactobacillus plantarum AKU 1009a was selected as a potential strain for CLA production from linoleic acid. Washed cells of L. plantarum with high levels of CLA production were obtained by cultivation in a nutrient medium with 0.06% (wt/vol) linoleic acid (cis-9,cis-12-octadecadienoic acid). Under the optimal reaction conditions with the free form of linoleic acid as the substrate, washed cells of L. plantarum produced 40 mg CLA/mL reaction mixture (33% molar yield) from 12% (wt/vol) linoleic acid in 108 h. The resulting CLA was a mixture of two CLA isomers, cis-9,trans-11 (or trans-9,cis-11)-octadecadienoic acid (CLA1, 38% of total CLA) and trans-9,trans-11-octadecadienoic acid (CLA2, 62% of total CLA), and accounted for 50% of the total FA obtained. A higher yield (80% molar yield to linoleic acid) was attained with 2.6% (wt/vol) linoleic acid as the substrate in 96 h, resulting in CLA production of 20 mg/mL reaction mixture [consisting of CLA1 (2%) and CLA2 (98%)] and accounting for 80% of total FA obtained. Most of the CLA produced was associated with the cells (ca. 380 mg CLA/g dry cells), mainly as FFA.

Key words

Conjugated linoleic acidCLAlactic acid bacteriaLactobacillusLactobacillus plantarumlinoleic acid

Copyright information

© AOCS Press 2002

Authors and Affiliations

  • Shigenobu Kishino
    • 1
  • Jun Ogawa
    • 1
  • Yoriko Omura
    • 1
  • Kenji Matsumura
    • 1
  • Sakayu Shimizu
    • 1
  1. 1.Division of Applied Life Sciences, Graduate School of AgricultureKyoto UniversityKyotoJapan