Journal of the American Oil Chemists' Society

, Volume 78, Issue 9, pp 941–947

Transgenic cotton plants with increased seed oleic acid content

  • Kent D. Chapman
  • Shea Austin-Brown
  • Salvatore A. Sparace
  • Anthony J. Kinney
  • Kevin G. Ripp
  • Irma L. Pirtle
  • Robert M. Pirtle
Article

DOI: 10.1007/s11746-001-0368-y

Cite this article as:
Chapman, K.D., Austin-Brown, S., Sparace, S.A. et al. J Amer Oil Chem Soc (2001) 78: 941. doi:10.1007/s11746-001-0368-y

Abstract

Cottonseed typically contains about 15% oleic acid. Here we report the development of transgenic cotton plants with higher seed oleic acid levels. Plants were generated by Agrobacterium-mediated transformation. A binary vector was designed to suppress expression of the endogenous cottonseed †-12 desaturase (fad2) by subcloning a mutant allele of a rapeseed fad2 gene downstream from a heterologous, seedspecific promoter (phaseolin). Fatty acid profiles of total seed lipids from 43 independent transgenic lines were analyzed by gas chromatography. Increased seed oleic acid content ranged from 21 to 30% (by weight) of total fatty acid content in 22 of the primary transformants. The increase in oleic acid content was at the expense of linoleic acid, consistent with reduced activity of cottonseed FAD2. Progeny of some lines yielded oleic acid content as high as 47% (three times that of standard cottonseed oil). Molecular analyses of nuclear DNA from transgenics confirmed the integration of the canola transgene into the cotton genome. Collectively, our results extend the metabolic engineering of vegetable oils to cottonseed and should provide the basis for the development of a family of novel cottonseed oils.

Key Words

Cottonseed oilfatty acid metabolismmetabolic engineeringoilseeds

Copyright information

© AOCS Press 2001

Authors and Affiliations

  • Kent D. Chapman
    • 1
  • Shea Austin-Brown
    • 1
  • Salvatore A. Sparace
    • 2
  • Anthony J. Kinney
    • 3
  • Kevin G. Ripp
    • 3
  • Irma L. Pirtle
    • 1
  • Robert M. Pirtle
    • 1
  1. 1.Division of Biochemistry and Molecular Biology, Cottonseed Development GroupUniversity of North Texas Department of Biological SciencesDentoh
  2. 2.Department of Plant SciencesMcGill University, McDonald CampusQuébecCanada
  3. 3.DuPont Experimental StationWilmington