Electron spin resonance spectroscopy for determination of the oxidative stability of food lipids
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Evaluation of the oxidative stability of food lipids based on the tendency of formation of radicals is shown to be possible using electron spin resonance (ESR) spectroscopy and the spin-trapping technique. Induction time can be determined for mildly accelerated conditions (50°C for lipid fraction from mayonnaise enriched with fish oil), and the length of the induction time decreases during storage and γ-tocopherol depletion. The protection by ethylenediaminetetraacetic acid against initiation of lipid oxidation is also detected in the new assay. For more oxidatively stable lipids (butter, rapeseed oil, dairy spread) the mildly accelerated conditions can be used in the assay, provided that difference in signal height for fixed times replaces determination of induction time. ESR spin trapping provides a sensitive method for evaluating the oxidative stability of food lipids. Detection of radicals in the lipid as an early event in oxidation allows mild conditions to be used, and future experiments should also include sensory evaluation in relation to determination of practical shelf life.
- Frankel, F.N., In Search of Better Methods to Evaluate Natural Antioxidants and Oxidative Stability in Food Lipids, Trends Food Sci. Technol. 4:220–225 (1993). CrossRef
- Wan, P.J., Accelerated Stability Methods, in Methods to Assess Quality and Stability of Oils and Fat-Containing Foods, edited by K. Warner and N.A.M. Eskin, AOCS Press, Champaign, 1995, pp. 179–189.
- Uchida, M., and M. Ono. Improvement for Oxidative Flavor Stability of Beer—Rapid Prediction Method for Beer Flavor Stability by Electron Spin Resonance Spectroscopy, J. Am. Soc. Brew. Chem. 54:205–222 (1996).
- Andersen, M.L., and L.H. Skibsted, Electron Spin-Resonance Spin Trapping Identification of Radicals Formed During Aerobic Forced Aging of Beer, J. Agric. Food Chem. 46:1272–1275 (1998). CrossRef
- Stapelfeldt, H., B.R. Nielsen, and L.H. Skibsted, Towards Use of Electron Spin Resonance Spectrometry in Quality Control of Milk Powder. Correlation Between Sensory Score of Instant Whole Milk Powders and Concentration of Free Radicals and 2-Thiobarbituric Acid Reactive Substances, Milchwissenschaft 52:682–685 (1997).
- Kristensen, D., and L.H. Skibsted, Comparison of Three Methods Based on Electron Spin Resonance Spectrometry for Evaluation of Oxidative Stability of Processed Cheese, J. Agric. Food Chem. 47:3099–3104 (1999). CrossRef
- Vicente, L., N. Deighton, S.M. Glidewell, J.A. Empis, and B.A. Goodman, In Situ Measurement of Free Radical Formation During the Thermal Decomposition of Grape Seed Oil Using “Spin Trapping” and Electron Paramagnetic Resonance Spectroscopy, Z. Lebensm. Unters. Forsch. 200:44–46 (1995). CrossRef
- Thomsen, M.K., H. Vedstesen, and L.H. Skibsted, Quantification of Radical Formation in Oil-in-Water Food Emulsions by Electron Spin Resonance Spectroscopy, J. Food Lipids. 6:149–158 (1999).
- FIL-IDF 74, Anhydrous Fat, Determination of Peroxide Value, International Dairy Federation, Brussels, Belgium, 1991.
- Meyer, A.S., and C. Jacobsen, Fate of the Synergistic Antioxidants System Ascorbic Acid, Lecithin and Tocopherol in Mayonnaise: Partition of Ascorbic Acid, J. Food Lipids 3:139–148 (1996).
- Thomsen, M.K., C. Jacobsen, and L.H. Skibsted, Mechanism of Initiation of Oxidation in Mayonnaise Enriched with Fish Oil as Studied by Electron Spin Resonance Spectroscopy, Eur. Food Res. Tech. (2000), in press.
- Christensen, T.C., and G. Hølmer, Lipid Oxidation Determination in Butter and Dairy Spreads by HPLC, J. Food Sci. 61:486–489 (1996). CrossRef
- St. Laurent, A.-M., M. Hidiroglou, M. Snoddon, and J.W.G. Nicholson, Effect of α-Tocopherol Supplementation to Dairy Cows on Milk and Plasma α-Tocopherol Concentrations and on Spontaneous Oxidized Flavor in Milk, Can. J. Anim. Sci. 70: 561–570 (1990). CrossRef
- Griffith, O.H., P.J. Dehlinger, and S.P. Van, Shape of the Hydrophobic Barrier of Phospholipids Bilayers (evidence for water penetration in biological membranes), J. Membr. Biol. 15:159–192 (1974). CrossRef
- Buettner, G.R., Spin Trapping: ESR Parameters of Spin Adducts, Free Radicals Biol. Med. 3:259–303 (1987).
- Andersson, K., and H. Lingnert, Influence of Oxygen and Copper Concentration on Lipid Oxidation in Rapeseed Oil, J. Am. Oil Chem. Soc. 75:1041–1046 (1998).
- Lampi, A.-M., L. Kataja, A. Kamal-Eldin, and P. Vieno, Antioxidant Activities of α- and γ-Tocopherols in the Oxidation of Rapeseed Oil Triacylglycerols, ——Ibid. 76:749–755 (1999).
- Shantha, N.C., and E.A. Decker, Rapid, Sensitive, Iron-Based Spectrophotometric Methods for Determination of Peroxide Values of Food Lipids, J. AOAC Int. 77:421–424 (1994).
- Electron spin resonance spectroscopy for determination of the oxidative stability of food lipids
Journal of the American Oil Chemists' Society
Volume 77, Issue 7 , pp 725-730
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- Electron spin resonance
- food lipids
- lipid oxidation
- peroxide value
- spin trapping
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