Lipids

, Volume 34, Issue 9, pp 915–920

Eicosapentaenoic acid and docosahexaenoic acid selectively attenuate U46619-induced smooth muscle cell proliferation

  • Rajbabu Pakala
  • Rajashree Pakala
  • Claude Benedict
Article

DOI: 10.1007/s11745-999-0440-2

Cite this article as:
Pakala, R., Pakala, R. & Benedict, C. Lipids (1999) 34: 915. doi:10.1007/s11745-999-0440-2

Abstract

It is well known that vascular smooth muscle cell (SMC) proliferation is a key step in atheromatous plaque formation. Thromboxane A2 (T×A2), released from aggregating platelets and an injured vessel wall, may play an important role in the development of atheromatous plaque. Many animal studies have suggested that n−3 polyunsaturated fatty acids eicosapentaenoic acid (EPA, 20∶5n−3) and docosahexaenoic acid (DHA, 22∶6n−3) present in the fish oils have antiatherosclerotic effects. In the present study, we investigated the effect of EPA and DHA on I×A2-induced SMC proliferation. To determine the functional selectivity of n−3 fatty acids, we also tested the effect of arachidonic acid (AA, 20∶4n−6), γ-linolenic acid) (INA, 18∶3n−6), and oleic acid (OA, 18∶1n−9) on T×A2-induced SMC proliferation. Only LPA and DHA prevented the SMC proliferation induced by the T×A2 mimetic U46619. When EPA and DHA were added together in the ratio in which they are present in menhaden oil, EPA and DHA acted synergistically to block the SMC proliferation induced by the TXA2-mimetic. These findings suggest that the n−3 polyunsaturated fatty acids in fish oils may exert antiatherosclerotic effects by blocking the mitogenstimulated proliferation of SMC.

Abbreviations

AA

arachidonic acid

DHA

docosahexaenoic acid

DMEM

Dulbecco’s modified Eagle’s medium

EPA

eicosapentaenoic acid

FBS

fetal bovine serum

IP

inositol triphosphate

LNA

γ-linolenic acid

OA

oleic acid

PBS

phosphate-buffered saline

PUFA

polyunsaturated fatty acids

SMC

smooth muscle cell

T×A2

thromboxane A2

Copyright information

© AOCS Press 1999

Authors and Affiliations

  • Rajbabu Pakala
    • 1
  • Rajashree Pakala
    • 1
  • Claude Benedict
    • 1
  1. 1.Department of Internal Medicine, Division of CardiologyUniversity of Texas HSC, Medical SchoolHonston