Lipids

, Volume 51, Issue 10, pp 1145–1152

Alpha-Lipoic Acid Alleviates Acute Inflammation and Promotes Lipid Mobilization During the Inflammatory Response in White Adipose Tissue of Mice

  • Jun Guo
  • Shixing Gao
  • Zhiqing Liu
  • Ruqian Zhao
  • Xiaojing Yang
Original Article

DOI: 10.1007/s11745-016-4185-2

Cite this article as:
Guo, J., Gao, S., Liu, Z. et al. Lipids (2016) 51: 1145. doi:10.1007/s11745-016-4185-2

Abstract

Recently, white adipose tissue has been shown to exhibit immunological activity, and may play an important role in host defense and protection against bacterial infection. Αlpha-lipoic acid (α-LA) has been demonstrated to function as an anti-inflammatory and anti-oxidant agent. However, its influence on the inflammatory response and metabolic changes in white adipose tissue remains unknown. We used male C57BL/6 mice as models to study the effect of α-LA on the inflammatory response and metabolic changes in white adipose tissue after stimulation with lipopolysaccharide (LPS). The non-esterified fatty acid content was measured by an automatic biochemical analyzer. The expression of inflammation-, lipid- and energy metabolism-related genes and proteins was determined by quantitative real-time polymerase chain reaction and western blotting. The results indicated that α-LA significantly decreased the epididymis fat weight index and the non-esterified fatty acid content in plasma compared with the control group. LPS significantly increased the expression of inflammation genes and α-LA reduced their expression. The LPS-induced expression of nuclear factor-κB protein was decreased by α-LA. Regarding lipid metabolism, α-LA significantly counteracted the inhibitory effects of LPS on the expression of hormone-sensitive lipase gene and protein. α-LA evidently increased the gene expression of fatty acid transport protein 1 and cluster of differentiation 36. Regarding energy metabolism, α-LA significantly increased the expression of most of mitochondrial DNA-encoded genes compared with the control and LPS group. Accordingly, α-LA can alleviate acute inflammatory response and this action may be related with the promotion of lipid mobilization in white adipose tissue.

Keywords

Alpha-lipoic acid White adipose tissue Inflammation Metabolism 

Abbreviations

α-LA

Αlpha-lipoic acid

LPS

Lipopolysaccharide

WAT

White adipose tissue

NF-κB

Nuclear factor-κB

TNF-α

Tumor necrosis factor-α

TLR4

Toll-like receptor 4

CON

Control

NEFA

Non-esterified fatty acid

PPIA

Peptidylprolyl isomerase A

HSL

Hormone-sensitive lipase

ATGL

Adipose triglyceride lipase

COX1

Cytochrome c oxidase subunit 1

IL-1α

Interleukin-1α

PPARγ

Peroxisome proliferator-activated receptor γ

C/EBPβ

CAAT/enhancer-binding protein β

SREBP1

Sterol regulatory element-binding protein 1

ACACA

Acetyl-CoA carboxylase 1

FASN

Fatty acid synthase

SCD1

Stearoyl-CoA desaturase 1

FATP1

Fatty acid transport protein 1

CD36

Cluster of differentiation 36

CPT-1a

Carnitine palmitoyltransferase-1a

ND1

NADH dehydrogenase subunit 1

ATF6

ATP synthase F0 subunit 6

CYTB

Cytochrome b

mtDNA

Mitochondrial deoxyribonucleic acid

Copyright information

© AOCS 2016

Authors and Affiliations

  • Jun Guo
    • 1
  • Shixing Gao
    • 1
  • Zhiqing Liu
    • 1
  • Ruqian Zhao
    • 1
  • Xiaojing Yang
    • 1
  1. 1.Key Laboratory of Animal Physiology and BiochemistryNanjing Agricultural UniversityNanjingPeople’s Republic of China