Lipids

, Volume 48, Issue 7, pp 749–755

Interactions Between Oil Substrates and Glucose on Pure Cultures of Ruminal Lipase-Producing Bacteria

  • H. D. Edwards
  • R. C. Anderson
  • T. M. Taylor
  • R. K. Miller
  • M. D. Hardin
  • D. J. Nisbet
  • N. A. Krueger
  • S. B. Smith
Original Article

DOI: 10.1007/s11745-013-3793-3

Cite this article as:
Edwards, H.D., Anderson, R.C., Taylor, T.M. et al. Lipids (2013) 48: 749. doi:10.1007/s11745-013-3793-3

Abstract

The hydrolysis of free fatty acids from lipids is a prerequisite for biohydrogenation, a process that effectively saturates free fatty acids. Anaerovibrio lipolyticus 5s and Butyrivibrio fibrisolvens have long been thought to be the major contributors to ruminal lipolysis; however, Propionibacterium avidum and acnes recently have been identified as contributing lipase activity in the rumen. In order to further characterize the lipase activity of these bacterial populations, each was grown with three different lipid substrates, olive oil, corn oil, and flaxseed oil (3 %). Because different finishing rations contain varying levels of glycogen (a source of free glucose) this study also documented the effects of glucose on lipolysis. P. avidum and A. lipolyticus 5s demonstrated the most rapid rates (P < 0.05) of lipolysis for cultures grown with olive oil and flaxseed oil, respectively. A. lipolyticus, B. fibrisolvens, and P. avidum more effectively hydrolyzed flaxseed oil than olive oil or corn oil, especially in the presence of 0.02 % glucose. Conversely, P. acnes hydrolyzed corn oil more readily than olive oil or flaxseed oil and glucose had no effect on lipolytic rate. Thus, these bacterial species demonstrated different specificities for oil substrates and different sensitivities to glucose.

Keywords

Lipid chemistryLipasesLipid metabolismFatty acid metabolism

Abbreviations

FFA

Free fatty acids

CLA

Conjugated linoleic acid

Copyright information

© AOCS 2013

Authors and Affiliations

  • H. D. Edwards
    • 1
  • R. C. Anderson
    • 1
  • T. M. Taylor
    • 2
  • R. K. Miller
    • 2
  • M. D. Hardin
    • 2
    • 3
  • D. J. Nisbet
    • 1
  • N. A. Krueger
    • 1
  • S. B. Smith
    • 2
  1. 1.United States Department of Agriculture/Agricultural Research Service, Southern PlainsCollege StationUSA
  2. 2.Department of Animal Science2471 Texas A&M UniversityCollege StationUSA
  3. 3.Agricultural Research CenterFood and Feed Safety Research UnitCollege StationUSA