Lipids

, Volume 48, Issue 6, pp 557–567

Egg Consumption Modulates HDL Lipid Composition and Increases the Cholesterol-Accepting Capacity of Serum in Metabolic Syndrome

  • Catherine J. Andersen
  • Christopher N. Blesso
  • Jiyoung Lee
  • Jacqueline Barona
  • Dharika Shah
  • Michael J. Thomas
  • Maria Luz Fernandez
Original Article

DOI: 10.1007/s11745-013-3780-8

Cite this article as:
Andersen, C.J., Blesso, C.N., Lee, J. et al. Lipids (2013) 48: 557. doi:10.1007/s11745-013-3780-8

Abstract

We recently demonstrated that daily whole egg consumption during moderate carbohydrate restriction leads to greater increases in plasma HDL-cholesterol (HDL-C) and improvements in HDL profiles in metabolic syndrome (MetS) when compared to intake of a yolk-free egg substitute. We further investigated the effects of this intervention on HDL composition and function, hypothesizing that the phospholipid species present in egg yolk modulate HDL lipid composition to increase the cholesterol-accepting capacity of subject serum. Men and women classified with MetS were randomly assigned to consume either three whole eggs (EGG, n = 20) per day or the equivalent amount of egg substitute (SUB, n = 17) throughout a 12-week moderate carbohydrate-restricted (25–30 % of energy) diet. Relative to other HDL lipids, HDL-cholesteryl ester content increased in all subjects, with greater increases in the SUB group. Further, HDL-triacylglycerol content was reduced in EGG group subjects with normal baseline plasma HDL-C, resulting in increases in HDL-CE/TAG ratios in both groups. Phospholipid analysis by mass spectrometry revealed that HDL became enriched in phosphatidylethanolamine in the EGG group, and that EGG group HDL better reflected sphingomyelin species present in the whole egg product at week 12 compared to baseline. Further, macrophage cholesterol efflux to EGG subject serum increased from baseline to week 12, whereas no changes were observed in the SUB group. Together, these findings suggest that daily egg consumption promotes favorable shifts in HDL lipid composition and function beyond increasing plasma HDL-C in MetS.

Keywords

HDLPhospholipidsCholesterol effluxMetabolic syndromeEggsCarbohydrate-restricted diet

Abbreviations

ABCA1

ATP-binding cassette transporter A1

ABCG1

ATP-binding cassette transporter G1

BCA

Bicinchoninic acid

CAD

Coronary artery disease

CE

Cholesteryl ester

CerPCho

Sphingomyelin

CETP

Cholesteryl ester transfer protein

CVD

Cardiovascular disease

DMPC

Dimyristoylphosphatidylcholine

EGG

Whole egg group

FC

Free cholesterol

HDL-C

Plasma HDL-cholesterol

HDL-PL

HDL-phospholipids

LCAT

Lecithin-cholesterol acyltransferase

LysoPtdCho

Lysophosphatidylcholine

NCEP ATP III

National Cholesterol Education Program Adult Treatment Panel III

MetS

Metabolic syndrome

PL

Phospholipid

PtdCho

Phosphatidylcholine

PtdEtn

Phosphatidylethanolamine

PtdIns

Phosphatidylinositol

RCT

Reverse cholesterol transport

SDS

Sodium dodecyl sulfate

SR-BI

Scavenger receptor class B I

SUB

Egg yolk-free egg substitute group

TAG

Triacylglycerol

Copyright information

© AOCS 2013

Authors and Affiliations

  • Catherine J. Andersen
    • 1
  • Christopher N. Blesso
    • 1
    • 3
  • Jiyoung Lee
    • 1
  • Jacqueline Barona
    • 1
    • 4
  • Dharika Shah
    • 2
  • Michael J. Thomas
    • 2
  • Maria Luz Fernandez
    • 1
  1. 1.Department of Nutritional SciencesUniversity of ConnecticutStorrsUSA
  2. 2.Biochemistry, Wake Forest School of MedicineWinston-SalemUSA
  3. 3.Pathology, Wake Forest School of MedicineWinston-SalemUSA
  4. 4.School of MicrobiologyUniversity of AntioquiaMedellinColombia