Lipids

, Volume 48, Issue 3, pp 209–218

Porcine G0/G1 Switch Gene 2 (G0S2) Expression is Regulated During Adipogenesis and Short-Term In-Vivo Nutritional Interventions

Authors

  • Jinsoo Ahn
    • Department of Animal SciencesThe Ohio State University
    • The Ohio State University Interdisciplinary Ph.D. Program in NutritionThe Ohio State University
  • Shin-Ae Oh
    • Department of Animal SciencesThe Ohio State University
  • Yeunsu Suh
    • Department of Animal SciencesThe Ohio State University
  • Steven J. Moeller
    • Department of Animal SciencesThe Ohio State University
    • Department of Animal SciencesThe Ohio State University
    • The Ohio State University Interdisciplinary Ph.D. Program in NutritionThe Ohio State University
Original Article

DOI: 10.1007/s11745-013-3756-8

Cite this article as:
Ahn, J., Oh, S., Suh, Y. et al. Lipids (2013) 48: 209. doi:10.1007/s11745-013-3756-8

Abstract

Adipose triglyceride lipase (ATGL), catalyzing the initial step of hydrolysis of triacylglycerol (TAG) in adipocytes, has been known to be inhibited by G0/G1 switch gene 2 (G0S2). In this study, we report the porcine G0S2 cDNA and amino acid sequences as well as the expression level of porcine G0S2. The porcine G0S2 mRNA was abundantly expressed in adipose tissue and liver among various tissues. In adipose tissue, porcine G0S2 expression was 16-fold higher in the fat cell fraction than the stromal vascular fraction. The G0S2 level increased significantly during adipogenesis in vitro and in vivo. These data indicate that G0S2 expression is closely associated with lipid accumulation and adipogenesis. Considering G0S2 as an inhibitor of cell proliferation, the relatively low levels of G0S2 in preadipocytes and adipose tissues of fetal and neonatal pigs compared to adipocytes and adipose tissues of adult pigs may allow the fast cell proliferation rates. Further studies showed that a short-term 24-h fast down-regulated G0S2 expression and increased ATGL expression in adipose tissue; however, a long-term calorie restriction for 8 days had no influence on the level of G0S2 but increased ATGL expression. Therefore, porcine G0S2, which is both a negative regulator of ATGL-mediated lipolysis and cell proliferation in adipose tissue, can be down-regulated in vivo by a short-term 24-h fast followed by increased ATGL-mediated lipolysis.

Keywords

Adipose triglyceride lipase (ATGL) Lipolysis Triacylglycerol (TAG) G0/G1 switch gene 2 (G0S2) Porcine Adipocyte Differentiation Adipose tissue Fast Calorie restriction

Abbreviations

AA

Amino acid

ATGL

Adipose triglyceride lipase

DAG

Diacylglycerol

DLK1

Delta-like 1 homolog

FBS

Fetal bovine serum

FFA

Free fatty acids

G0S2

G0/G1 switch gene 2

HSL

Hormone sensitive lipase

LPL

Lipoprotein lipase

MAG

Monoacylglycerol

NEFA

Nonesterified fatty acids

PPARγ

Peroxisome proliferator-activated receptor gamma

RT-PCR

Reverse transcription polymerase chain reaction

SV

Stromal vascular

TAG

Triacylglycerol

Supplementary material

11745_2013_3756_MOESM1_ESM.pdf (84 kb)
Multiple alignments of complete coding sequences of G0S2 from Human (GenBank accession NM_015714), Mouse (GenBank accession NM_008059) Berkshire (GenBank accession JQ_013998), Landrace (GenBank accession JQ_013999), and Duroc (GenBank accession JQ_014000). Identities to the human G0S2 nucleotide sequence are denoted by a period, and nucleotide differences among porcine species were indicated by an open box (PDF 83 kb)

Copyright information

© AOCS 2013