Lipids

, Volume 47, Issue 2, pp 185–193

Steryl Glucoside and Acyl Steryl Glucoside Analysis of Arabidopsis Seeds by Electrospray Ionization Tandem Mass Spectrometry

  • Kathrin Schrick
  • Sunitha Shiva
  • James C. Arpin
  • Nicole Delimont
  • Giorgis Isaac
  • Pamela Tamura
  • Ruth Welti
Original Article

DOI: 10.1007/s11745-011-3602-9

Cite this article as:
Schrick, K., Shiva, S., Arpin, J.C. et al. Lipids (2012) 47: 185. doi:10.1007/s11745-011-3602-9

Abstract

Establishment of sensitive methods for the detection of cellular sterols and their derivatives is a critical step in developing comprehensive lipidomics technology. We demonstrate that electrospray ionization tandem (triple quadrupole) mass spectrometry (ESI-MS/MS) is an efficient method for monitoring steryl glucosides (SG) and acyl steryl glucosides (ASG). Comparison of analysis of SG and ASG by ESI-MS/MS with analysis by gas chromatography with flame ionization detection (GC–FID) shows that the two methods yield similar molar compositions. These data demonstrate that ESI-MS/MS response per molar amount of sterol conjugate is similar among various molecular species of SG and ASG. Application of ESI-MS/MS to seed samples from wild-type Arabidopsis and a mutant deficient in two UDP-glucose:sterol glucosyltransferases, UGT80A2 and UGT80B1, revealed new details on the composition of sitosteryl, campesteryl and stigmasteryl glucosides and ASG. SG were decreased by 86% in the ugt80A2,B1 double mutant, compared to the wild-type, while ASG were reduced 96%. The results indicate that these glucosyltransferases account for much of the accumulation of the sterol conjugates in wild-type Arabidopsis seeds.

Keywords

Steryl glucosidesAcyl steryl glucosidesSterolsElectrospray ionization tandem mass spectrometryGas chromatographyArabidopsis thalianaUDP-glucose:sterol glucosyltransferaseugt80A2,B1, UGT80A2, UGT80B1

Abbreviations

SG

Steryl glucosides

ASG

Acyl steryl glucosides

ESI-MS/MS

Electrospray ionization tandem (triple quadrupole) mass spectrometry

GC

Gas chromatography

FID

Flame ionization detection

Supplementary material

11745_2011_3602_MOESM1_ESM.doc (82 kb)
Supplementary material 2 (DOC 83 kb)
11745_2011_3602_MOESM2_ESM.doc (38 kb)
Supplementary material 3 (DOC 39 kb)

Copyright information

© AOCS 2011

Authors and Affiliations

  • Kathrin Schrick
    • 1
  • Sunitha Shiva
    • 1
  • James C. Arpin
    • 1
  • Nicole Delimont
    • 1
  • Giorgis Isaac
    • 1
  • Pamela Tamura
    • 1
  • Ruth Welti
    • 1
  1. 1.Division of Biology116 Ackert Hall, Kansas State UniversityManhattanUSA