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Dietary Conjugated Linoleic Acid Induces Lipolysis in Adipose Tissue of Coconut Oil-Fed Mice but not Soy Oil-Fed Mice

  • Original Article
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Lipids

Abstract

Mice fed diets containing conjugated linoleic acid (CLA) are leaner than mice not fed CLA. This anti-obesity effect is amplified in mice fed coconut oil-containing or fat free diets, compared to soy oil diets. The present objective was to determine if CLA alters lipolysis in mice fed different base oils. Mice were fed diets containing soy oil (SO), coconut oil (CO), or fat free (FF) for 6 weeks, followed by 10 or 12 days of CLA or no CLA supplementation. Body fat, tissue weights, and ex vivo lipolysis were determined. Relative protein abundance and activation of perilipin, hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL), and adipose differentiation related protein (ADRP) were determined by western blotting. CLA feeding caused mice to have less (P < 0.05) body fat than non-CLA fed mice. This was enhanced in CO and FF-fed mice (CLA × oil source, P < 0.05). There was also a CLA × oil source interaction on lipolysis as CO + CLA and FF + CLA-fed mice had increased (P < 0.05) rates of lipolysis but SO + CLA-fed mice did not. However, after 12 days of CLA consumption, activated perilipin was increased (P < 0.05) only in SO + CLA-fed mice and total HSL and ATGL were decreased (P < 0.05) in CO + CLA-fed mice. Therefore, the enhanced CLA-induced body fat loss in CO and FF-fed mice appears to involve increased lipolysis but this effect may be decreasing by 12 days of CLA consumption.

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Abbreviations

ADRP:

Adipose differentiation related protein

ATGL:

Adipose triglyceride lipase

CGI-58:

Comparative gene identification-58

CLA:

Conjugated linoleic acid (18:2)

CO:

Coconut oil

FF:

Fat free

HSL:

Hormone sensitive lipase

LNA:

Linoleic acid

LD:

Lipid droplets

NEFA:

Non esterified fatty acids

PKA:

Protein kinase A

SO:

Soy oil

TNF-α:

Tumor necrosis factor α

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Authors and Affiliations

  1. Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, 6108, USA

    S. Ippagunta & K. M. Hargrave-Barnes

  2. Department of Animal Science, University of Nebraska, Lincoln, NE, 68583, USA

    T. J. Hadenfeldt & J. L. Miner

Authors
  1. S. Ippagunta
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  2. T. J. Hadenfeldt
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  3. J. L. Miner
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  4. K. M. Hargrave-Barnes
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Correspondence to K. M. Hargrave-Barnes.

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Ippagunta, S., Hadenfeldt, T.J., Miner, J.L. et al. Dietary Conjugated Linoleic Acid Induces Lipolysis in Adipose Tissue of Coconut Oil-Fed Mice but not Soy Oil-Fed Mice. Lipids 46, 821–830 (2011). https://doi.org/10.1007/s11745-011-3574-9

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  • DOI: https://doi.org/10.1007/s11745-011-3574-9

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