, Volume 46, Issue 9, pp 821–830

Dietary Conjugated Linoleic Acid Induces Lipolysis in Adipose Tissue of Coconut Oil-Fed Mice but not Soy Oil-Fed Mice

  • S. Ippagunta
  • T. J. Hadenfeldt
  • J. L. Miner
  • K. M. Hargrave-Barnes
Original Article

DOI: 10.1007/s11745-011-3574-9

Cite this article as:
Ippagunta, S., Hadenfeldt, T.J., Miner, J.L. et al. Lipids (2011) 46: 821. doi:10.1007/s11745-011-3574-9


Mice fed diets containing conjugated linoleic acid (CLA) are leaner than mice not fed CLA. This anti-obesity effect is amplified in mice fed coconut oil-containing or fat free diets, compared to soy oil diets. The present objective was to determine if CLA alters lipolysis in mice fed different base oils. Mice were fed diets containing soy oil (SO), coconut oil (CO), or fat free (FF) for 6 weeks, followed by 10 or 12 days of CLA or no CLA supplementation. Body fat, tissue weights, and ex vivo lipolysis were determined. Relative protein abundance and activation of perilipin, hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL), and adipose differentiation related protein (ADRP) were determined by western blotting. CLA feeding caused mice to have less (P < 0.05) body fat than non-CLA fed mice. This was enhanced in CO and FF-fed mice (CLA × oil source, P < 0.05). There was also a CLA × oil source interaction on lipolysis as CO + CLA and FF + CLA-fed mice had increased (P < 0.05) rates of lipolysis but SO + CLA-fed mice did not. However, after 12 days of CLA consumption, activated perilipin was increased (P < 0.05) only in SO + CLA-fed mice and total HSL and ATGL were decreased (P < 0.05) in CO + CLA-fed mice. Therefore, the enhanced CLA-induced body fat loss in CO and FF-fed mice appears to involve increased lipolysis but this effect may be decreasing by 12 days of CLA consumption.


Conjugated linoleic acid (CLA)Specific LipidsDietary fatNutritionHormone sensitive lipaseLipases



Adipose differentiation related protein


Adipose triglyceride lipase


Comparative gene identification-58


Conjugated linoleic acid (18:2)


Coconut oil


Fat free


Hormone sensitive lipase


Linoleic acid


Lipid droplets


Non esterified fatty acids


Protein kinase A


Soy oil


Tumor necrosis factor α

Copyright information

© AOCS 2011

Authors and Affiliations

  • S. Ippagunta
    • 1
  • T. J. Hadenfeldt
    • 2
  • J. L. Miner
    • 2
  • K. M. Hargrave-Barnes
    • 1
  1. 1.Division of Animal and Nutritional SciencesWest Virginia UniversityMorgantownUSA
  2. 2.Department of Animal ScienceUniversity of NebraskaLincolnUSA