Lipids

, Volume 46, Issue 5, pp 399–407

Oral Docosapentaenoic Acid (22:5n-3) Is Differentially Incorporated into Phospholipid Pools and Differentially Metabolized to Eicosapentaenoic Acid in Tissues from Young Rats

Authors

    • Department of Human Biology and Nutritional Sciences, Animal Science and Nutrition BuildingUniversity of Guelph
  • Patricia Swidinsky
    • Department of Human Biology and Nutritional Sciences, Animal Science and Nutrition BuildingUniversity of Guelph
  • Eek Park
    • Department of Human Biology and Nutritional Sciences, Animal Science and Nutrition BuildingUniversity of Guelph
Original Article

DOI: 10.1007/s11745-011-3535-3

Cite this article as:
Holub, B.J., Swidinsky, P. & Park, E. Lipids (2011) 46: 399. doi:10.1007/s11745-011-3535-3
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Abstract

The present study assessed the effect of oral supplementation with docosapentaenoic acid (DPA, 22:5n-3) on the levels of serum and tissue lipid classes and their fatty acid compositions including individual phospholipid types in rat liver, heart, and kidney. Sprague–Dawley rats received daily oral gavage over 10 days as corn oil without (controls) or with purified DPA in free fatty acid form (21.2 mg/day). The DPA group exhibited significantly lower serum lipid concentrations. The concentrations in μmol/100 g serum or μmol/g tissue of DPA in the total lipid (TL) were higher by 2.3-, 2.4-, 10.9-, and 5.1-fold in the DPA group of serum, liver, heart, and kidney, respectively, with the phospholipids (PL) being the major DPA reservoir (45.2–52.1% of the DPA in the TL). No significant differences in DHA (22:6n-3) amounts in TL appeared. The highest relative mol% values as DPA were in heart tissue (means of 11.1% in PL and 16.2% in phosphatidylinositol) and lowest in kidney. The EPA (20:5n-3) concentrations were markedly higher in the DPA group and most pronounced in the kidney (5.1 times higher in the TL as compared to controls) relative to liver and heart yielding an estimated apparent % conversion of DPA to EPA of 67% and EPA:DPA ratios reaching 5.74 in kidney phosphatidylethanolamine. The serum lipid-lowering potential of dietary DPA and its impact in the kidney with the derived EPA warrants investigation.

Keywords

Docosapentaenoic acid (DPA 22:5n-3)Rat serumCholesteryl estersTriacylglycerol(s)LiverHeartKidneyApparent retroconversionEicosapentaenoic acid (EPA 20:5n-3)Individual phospholipids

Abbreviations

ARA

Arachidonic acid 20:4n-6

ACAT

Acyl-CoA:cholesterol acyltransferase

CE

Cholesteryl esters

CerPCho

Sphingomyelin

DHA

Docosahexaenoic acid 22:6n-3

DPA

Docosapentaenoic acid 22:5n-3

EPA

Eicosapentaenoic acid 20:5n-3

LCAT

Lecithin:cholesterol acyltransferase

NEFA

Non-esterified fatty acids

PL

Phospholipid(s)

PtdCho

Phosphatidylcholine

PtdEtn

Phosphatidylethanolamine

PtdIns

Phosphatidylinositol

PtdSer

Phosphatidylserine

PUFA

Polyunsaturated fatty acid

TAG

Triacylglycerol(s)

TC

Total cholesterol

TL

Total lipid(s)

TLC

Thin-layer chromatography

Copyright information

© AOCS 2011