Original Article

Lipids

, Volume 46, Issue 6, pp 487-494

First online:

Temporary Increase of PPAR-γ and Transient Expression of UCP-1 in Stromal Vascular Fraction Isolated Human Adipocyte Derived Stem Cells During Adipogenesis

  • Seong Jin JoAffiliated withDepartment of Dermatology, Seoul National University College of Medicine
  • , Won Woo ChoiAffiliated withWells Dermatology Clinic
  • , Eun Seong LeeAffiliated withCenter for NanoBio Convergence, Korea Research Institute of Standards and Science
  • , Jae Yong LeeAffiliated withCenter for NanoBio Convergence, Korea Research Institute of Standards and Science
  • , Hyun Sun ParkAffiliated withDepartment of Dermatology, Seoul National University College of Medicine
  • , Dae Won MoonAffiliated withCenter for NanoBio Convergence, Korea Research Institute of Standards and Science
  • , Hee Chul EunAffiliated withDepartment of Dermatology, Seoul National University College of Medicine
  • , Jin Ho ChungAffiliated withDepartment of Dermatology, Seoul National University College of Medicine Email author 

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Abstract

In this study, cells from the stromal vascular fraction of human subcutaneous tissues were induced to differentiate toward adipose cells in vitro for 2 weeks. During adipogenic differentiation, we followed the chronological changes in their morphology with Coherent anti-Stokes Raman scattering (CARS) microscopy and checked the PPAR-γ and UCP-1 expression with RT-PCR. On day 4 after inducing adipogenic differentiation, CARS imaging showed multiple small lipid droplets (LD) distributed peripherally along the cellular membrane. PPAR-γ began to express at this time and increased until day 14 at a steady rate. On day 7, the cells appeared as brown adipocytes with numerous small LD throughout the cytoplasm, and the mRNA level of UCP-1 rose abruptly by 6- to 7-fold. After an additional 7 days, CARS imaging showed the development of a large LD, which is characteristic of white adipocytes, and the mRNA level of UCP-1 slumped significantly. These results demonstrate the possibility that ADSC pass through a brown adipocyte-like stage while differentiating into white adipocytes.

Keywords

Adipogenesis Adipose-derived stem cell UCP-1