Lipids

, Volume 46, Issue 2, pp 171–178

Maternal Intake of Fish Oil but not of Linseed Oil Reduces the Antibody Response in Neonatal Mice

  • Lotte Lauritzen
  • T. M. R. Kjær
  • T. Porsgaard
  • M. B. Fruekilde
  • H. Mu
  • H. Frøkiær
Original Article

DOI: 10.1007/s11745-010-3519-8

Cite this article as:
Lauritzen, L., Kjær, T.M.R., Porsgaard, T. et al. Lipids (2011) 46: 171. doi:10.1007/s11745-010-3519-8

Abstract

Dietary levels of n-3 PUFA are believed to influence the immune system. The importance of the source of n-3 PUFA is debated. This study addressed how the content and source of n-3 PUFA in the maternal diet influenced tissue FA composition and the immune response to ovalbumin (OVA) in mice pups. From the day of conception and throughout lactation, dams were fed diets containing 4% fat from linseed oil (LSO), fish oil (FO) or a n-3 PUFA-deficient diet (DEF). Pups were injected with OVA within 24 h of birth and sacrificed at weaning (day 21). Overall, the content of n-3 PUFA in milk, liver and spleen reflected the source and only minor differences were observed in brain phospholipid 22:6n-3. The source had only limited influence on the n-3 PUFA accretion in peripheral tissue, with most pronounced differences in the spleen. The marine PUFA-group had reduced levels of total OVA-specific antibodies and OVA-IgG1 titers in the pup blood, while the response in the LSO-group did not differ from that in the DEF-group. There were no statistical differences in the cytokine responses to OVA-stimulated splenocytes, but the decrease in IgG1 was paralleled by an increase in IFNγ-production and a decrease in IL-6-production. Our results indicate that maternal intake of FO, but not of LSO, changes the offspring’s antigen-specific response and potentially increases Th1-polarization.

Keywords

n-3 long-chain polyunsaturated fatty acidTissue fatty acid incorporationImmune maturationSensitizationDietProgramming

Abbreviations

DEF

The n-3 PUFA-deficient group

20:5n-3

Eicosapentaenoic acid (individual fatty acids are named by number of carbon atoms: number of double bonds followed by the position of the last double bond)

FO

The fish oil group

Ig

Immunoglobulin

IL

Interleukin

IFNγ

Interferon-γ

LCPUFA

Long-chain PUFA

LSO

The linseed oil-group

OVA

Ovalbumin

PC

Phosphatidylcholine

PE

Phosphatidylethanolamine

TAG

Triacylglycerol

Th

T-helper lymphocytes

TNFα

Tumor necrosis factor-α

Copyright information

© AOCS 2011

Authors and Affiliations

  • Lotte Lauritzen
    • 1
  • T. M. R. Kjær
    • 2
  • T. Porsgaard
    • 2
  • M. B. Fruekilde
    • 2
  • H. Mu
    • 3
  • H. Frøkiær
    • 4
  1. 1.Department of Human Nutrition, Faculty of Life SciencesUniversity of CopenhagenFrederiksberg CDenmark
  2. 2.Department of Systems BiologyTechnical University of DenmarkLyngbyDenmark
  3. 3.Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical SciencesUniversity of CopenhagenCopenhagenDenmark
  4. 4.Department of Basic Sciences and Environment, Faculty of Life SciencesUniversity of CopenhagenFrederiksbergDenmark