Lipids

, Volume 45, Issue 5, pp 401–407

Saponified Evening Primrose Oil Reduces Melanogenesis in B16 Melanoma Cells and Reduces UV-Induced Skin Pigmentation in Humans

Original Article

DOI: 10.1007/s11745-010-3405-4

Cite this article as:
Koo, JH., Lee, I., Yun, SK. et al. Lipids (2010) 45: 401. doi:10.1007/s11745-010-3405-4

Abstract

This study was conducted to determine whether saponified evening primrose oil (sap-EPO) has the potential for use as a whitening agent and to investigate its underlying mechanisms of action. In B16 melanoma cells, sap-EPO dose-dependently inhibited isobutylmethylxanthine-induced melanogenesis with no cytotoxicity. This decrease in melanin production was correlated with reduced enzyme activity and decreased mRNA and protein levels of tyrosinase. The mRNA levels of tyrosinase-related proteins 1 and 2 decreased in response to treatment with sap-EPO, indicating that it regulated tyrosinase at the transcriptional level. Expression of microphthalmia-associated transcription factor was also decreased by sap-EPO as evidenced by decreased mRNA and protein levels. Additionally, topical application of sap-EPO resulted in efficient whitening of UVB-induced hyperpigmentation of human skin. Taken together, these results suggest that sap-EPO has the potential for use as a cosmetic whitening agent.

Keywords

Saponification Evening primrose oil Melanogenesis B16 melanoma cells Skin UV 

Abbreviations

DHICA

5,6-Dihydroxyindole-2-carboxylic acid

EPO

Evening primrose oil

IBMX

Isobutylmethylxanthine

MITF

Microphthalmia-associated transcription factor

sap-EPO

Saponified evening primrose oil

TRP

Tyrosinase-related protein

Copyright information

© AOCS 2010

Authors and Affiliations

  1. 1.Department of Biochemistry, Medical School and Diabetes Research CenterChonbuk National UniversityJeonjuKorea
  2. 2.Department of Dermatology, Medical School and Diabetes Research CenterChonbuk National UniversityJeonjuKorea

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