Lipids

, Volume 45, Issue 1, pp 63–71

The Spectrum of Plant and Animal Sterols in Different Oil-Derived Intravenous Emulsions

Authors

    • Department of Pediatrics, S Orsola-MalpighiMedical School of Bologna
  • Germana Bersani
    • Department of Pharmacy, S Orsola-MalpighiMedical School of Bologna
  • Sara Tala
    • Department of Clinical Biochemistry, S Orsola-MalpighiMedical School of Bologna
  • Gabriele Grossi
    • Department of Clinical Biochemistry, S Orsola-MalpighiMedical School of Bologna
  • Cristina Puggioli
    • Department of Pharmacy, S Orsola-MalpighiMedical School of Bologna
  • Massimo Masi
    • Department of Pediatrics, S Orsola-MalpighiMedical School of Bologna
Original Article

DOI: 10.1007/s11745-009-3371-x

Cite this article as:
Forchielli, M.L., Bersani, G., Tala, S. et al. Lipids (2010) 45: 63. doi:10.1007/s11745-009-3371-x
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Abstract

Intravenous lipid constituents have different effects on various biological processes. Some of these effects are protective, while others are potentially adverse. Phytosterols, in particular, seem to be implicated with parenteral nutrition-associated cholestasis. The aim of this study is to determine the amount of plant and animal sterols present in lipid formulations derived from different oil sources. To this end, animal (cholesterol) and plant (β-sitosterol, campesterol, and stigmasterol) sterols in seven different commercially available intravenous lipid emulsions (ILEs) were quantified by capillary gas chromatography after performing a lipid extraction procedure. The two major constituents of the lipid emulsions were cholesterol (range 14–57% of total lipids) and β-sitosterol (range 24–55%), followed by campesterol (range 8–18%) and stigmasterol (range 5–16%). The fish oil-derived formulation was an exception, as it contained only cholesterol. The mean values of the different sterols were statistically different across ILEs (P = 0.0000). A large percentage of pairwise comparisons were also statistically significant (P = 0.000), most notably for cholesterol and stigmasterol (14 out of 21 for both), followed by campesterol (12 out 21) and β-sitosterol (11 out 21). In conclusion, most ILEs combined significant amounts of phytosterols and cholesterol. However, their phytosterols:cholesterol ratios were reversed compared to the normal human diet.

Keywords

PhytosterolsCholesterolIntravenous lipid emulsionsCholestasisGas chromatography

Abbreviations

ILEs

Intravenous lipid emulsions

TPN

Total parenteral nutrition

PNAC

Parenteral nutrition-associated cholestasis

LCT

Long-chain triglycerides

MCT

Medium-chain triglycerides

GC

Gas chromatography

cGC

Capillary gas chromatography

Copyright information

© AOCS 2009