Lipids

, Volume 43, Issue 10, pp 925–936

Women and Smokers Have Elevated Urinary F2-Isoprostane Metabolites: A Novel Extraction and LC–MS Methodology

  • Alan W. Taylor
  • Richard S. Bruno
  • Maret G. Traber
Original Article

DOI: 10.1007/s11745-008-3222-1

Cite this article as:
Taylor, A.W., Bruno, R.S. & Traber, M.G. Lipids (2008) 43: 925. doi:10.1007/s11745-008-3222-1

Abstract

F2-Isoprostanes (F2-IsoPs), regio- and stereoisomers of prostaglandin F (PGF), and urinary F2-IsoP metabolites including 2,3-dinor-5,6-dihydro-8-iso-PGF [2,3-dinor-8-iso-PGF (2,3-dinor-F1)] and 2,3 dinor-8-iso-PGF (2,3-dinor-F2), have all been used as biomarkers of oxidative stress. A novel method was developed to measure these biomarkers using a single solid phase extraction (SPE) cartridge, separation by HPLC, and detection by negative mode selected reaction monitoring (SRM) mass spectrometry (MS), using authentic standards of PGF; 8-iso-PGF; 2,3-dinor-F1 and 2,3-dinor-F2 to identify specific chromatographic peaks. The method was validated in a population of healthy, college-aged nonsmokers (n = 6 M/8F) and smokers (n = 6 M/5F). Urinary F2-IsoP concentrations were ~0.2–1.5 μg/g creatinine, 2,3-dinor-F1 was ~1–3 μg/g and 2,3-dinor-F2 was ~3–5 μg/g. Additional F2-IsoPs metabolites were identified using SRM. The sum of all urinary F2-IsoP metabolites was 50–100 μg/g creatinine indicating their greater abundance than F2-IsoPs. Women had higher F2-IsoP metabolite concentrations than did men (MANOVA, main effect P = 0.003); cigarette smokers had higher concentrations than did nonsmokers (main effect P = 0.036). For men or women, respectively, smokers had higher metabolite concentrations than did nonsmokers (P < 0.05). Thus, our method simultaneously allows measurement of urinary F2-IsoPs and their metabolites for the determination of oxidative stress.

Keywords

Oxidative stress LC–MS F2-Isoprostanes F2-Isoprostane metabolites Prostaglandin F (PGFCigarette smoking Gender difference 2,3-dinor-5,6-dihydro-8-iso-PGF 2,3 dinor-8-iso-PGF 

Abbreviations

CE

Collision energies

F2-IsoPs

F2-Isoprostanes

GC–MS

Gas chromatography-mass spectrometry

LC–MS

Liquid chromatography-mass spectrometry

SPE

Solid phase extraction

SRM

Selected reaction monitoring

2,3 dinor-F1

2,3-dinor-5,6-dihydro-8-iso-PGF, or 2,3-dinor-8-iso-PGF

2,3 dinor-F2

2,3-dinor-8-iso-PGF

Copyright information

© AOCS 2008

Authors and Affiliations

  • Alan W. Taylor
    • 1
  • Richard S. Bruno
    • 2
  • Maret G. Traber
    • 1
  1. 1.Linus Pauling InstituteOregon State UniversityCorvallisUSA
  2. 2.Department of Nutritional SciencesUniversity of ConnecticutStorrsUSA