Lipids

, 43:663

Vitamin E Transfer from Lipid Emulsions to Plasma Lipoproteins: Mediation by Multiple Mechanisms

  • M. Hacquebard
  • M. Vandenbranden
  • W. J. Malaisse
  • J. M. Ruysschaert
  • R. J. Deckelbaum
  • Y. A. Carpentier
Original Article

DOI: 10.1007/s11745-008-3184-3

Cite this article as:
Hacquebard, M., Vandenbranden, M., Malaisse, W.J. et al. Lipids (2008) 43: 663. doi:10.1007/s11745-008-3184-3

Abstract

The present study determined alpha-tocopherol mass transfer from an alpha-tocopherol-rich emulsion to LDL and HDL, and assessed the potential of different mechanisms to modulate alpha-tocopherol transfers. Emulsion particles rich in alpha-tocopherol were incubated in vitro with physiological concentrations of LDL or HDL. The influence of plasma proteins was assessed by adding human lipoprotein poor plasma (LPP) fraction with intact vs heat inactivated PLTP, or with a specific cholesteryl ester transfer protein (CETP) inhibitor, or by adding purified PLTP or pig LPP which lacks CETP activity. After 4 h incubation in absence of LPP, alpha-tocopherol content was increased by ~80% in LDL and ~160% in HDL. Addition of LPP markedly enhanced alpha-tocopherol transfer leading to 350–400% enrichment in LDL or HDL at 4 h. Higher (~10 fold) enrichment was achieved after 20 h incubation with LPP. Facilitation of alpha-tocopherol transfer was (i) more than 50% higher with human vs pig LPP (despite similar PLTP phospholipid transfer activity), (ii) reduced by specific CETP activity inhibition, (iii) not fully suppressed by heat inactivation, and (iv) not restored by purified PLTP. In conclusion, alpha-tocopherol content in LDL and HDL can be markedly raised by rapid transfer from an alpha-tocopherol-rich emulsion. Our results indicate that alpha-tocopherol mass transfer between emulsion particles and lipoproteins is mediated by more than one single mechanism and that this transfer may be facilitated not only by PLTP but likely also by other plasma proteins such as CETP.

Keywords

Vitamin EAlpha-tocopherol mass transferLipid emulsionsLDLHDLPLTPCETP

Abbreviations

CETP

Cholesteryl ester transfer protein

HDL

High density lipoproteins

LDL

Low density lipoproteins

LPP

Lipoprotein poor plasma

PLTP

Phospholipid transfer protein

TC

Total cholesterol

TG

Triglycerides

TGRP

Triglyceride-rich particles

Copyright information

© AOCS 2008

Authors and Affiliations

  • M. Hacquebard
    • 1
  • M. Vandenbranden
    • 2
  • W. J. Malaisse
    • 1
  • J. M. Ruysschaert
    • 2
  • R. J. Deckelbaum
    • 3
  • Y. A. Carpentier
    • 1
  1. 1.L. Deloyers Laboratory for Experimental SurgeryUniversité Libre de BruxellesBrusselsBelgium
  2. 2.Laboratory of Structure and Function of Biological Membranes-Center of Structural Biology and BioinformaticsUniversité Libre de BruxellesBrusselsBelgium
  3. 3.Institute of Human NutritionColumbia UniversityNew YorkUSA