Differential biohydrogenation and isomerization of [U-13C]Oleic and [1-13C]Oleic acids by mixed ruminal microbes
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
The additional mass associated with 13C in metabolic tracers may interfere with their metabolism. The comparative isomerization and biohydrogenation of oleic, [1-13C]oleic, and [U-13C]oleic acids by mixed ruminal microbes was used to evaluate this effect. The percent of stearic, cis-14 and- 15, and trans-9 to-16 18∶1 originating from oleic acid was decreased for [U-13C]oleic acid compared with [1-13C]oleic acid. Conversely, microbial utilization of [U-13C]oleic acid resulted in more of the 13C label in cis-9 18∶1 compared with [1-13C]oleic acid (53.7 vs. 40.1%). The isomerization and biohydrogenation of oleic acid by ruminal microbes is affected by the mass of the labeled tracer.
- Wolfe, R.R. (1992) Radioactive and Stable Isotope Tracers in Biomedicine, Principles and Practice of Kinetic Analysis, Wiley-Liss, New York.
- Koletzko, B., Sauerwald, T., and Demmelmair, H. (1997) Safety of Stable Isotope Use. Eur. J. Pediatr. 156, S12-S17. CrossRef
- Hachey, D.L., Thomas, M.R., Emken, E.A., Garza, C., Brown-Booth, L., Adlof, R.O., and Klein, P.D (1987) Human Lactation: Maternal Transfer of Dietary Triglycerides Labeled with Stable Isotopes, J. Lipid Res. 28, 1185–1192.
- Demmelmair, H., Baumheuer, M., Koletxko, B., Dokoupil, K., and Kratl, G. (1998) Metabolism of U-13C-Labeled Linoleic Acid in Lactating Women. J. Lipid Res. 39, 1389–1396.
- Bell, P.M, Firth, R.G., and Rizza, R.A. (1986) Assessment of Insulin Action in Insulin-Dependent Diabetes Mellitus using [6-14C]Glucose, [3-3H]Glucose, and [2-3H]Glucose: Differences in the Apparent Pattern of Insulin Resistance Depending on the Isotope Used. J. Clin. Invest. 78, 1479–1486. CrossRef
- Argoud, G.M., Schade, D.S., and Eaton, R.P. (1987) Underestimation of Hepatic Glucose Production by Radioactive and Stable Tracers, Am. J. Physiol. 252, E606-E615.
- Chatzidakis, C., and Otto, D.A. (1987) Labeled Oxidation Products from [1-14C], [1-14C], and [16-14C]-Palmitate in Hepatocytes and Mitochondria, Lipids 22, 620–626. CrossRef
- Mosley, E.E., Powell, G.L., Riley, M.B., and Jenkins, T.C. (2002) Microbial Biohydrogenation of Oleic Acid to trans Isomers in vitro, J Lipid. Res. 43, 290–296.
- Harfoot, C.G., and Hazlewood, G.P. (1988) Lipid Metabolism in the Rumen, in The Rumen Microbial Ecosystem ed. P. N. Hobson, pp. 285–322. Elsevier Applied Science Publishers. London, [AUQ]
- Jenkins, T.C. (1993) Lipid Metabolism in the Rumen. J Dairy Sci. 76, 3851–3863. [AUQ] CrossRef
- Kramer, J.K.G., Fellner, V., Dugan, M.E.R., Sauer, F.D., Mossoba, M.M., and Yurawecz, M.P. (1997) Evaluating Acid and Base Catalysts in the Methylation of Milk and Rumen Fatty Acids with Special Emphasis on Conjugated Dienes and Total trans Fatty Acids, Lipids 32, 1219–1228. CrossRef
- Mosley, E.E., Shafii, B., Moate, P.J., and McGuire, M.A. (2006) Conjugated Linoleic Acid (cis-9, trans-11 CLA) Is Synthesized Directly from Vaccenic Acid in Lactating Dairy Cattle. J. Nutr. 136, 570–575.
- Proell, J.M., Mosley, E.E., Powell, G.L., and Jenkins, T.C. (2002) Isomerization of Stable Isotopically Labeled Elaidic Acid to cis and trans Monoenes by Ruminal Microbes. J. Lipid Res. 43, 2072–2076. CrossRef
- Palmquist, D.L., and Mattos, W. (1978) Turnover of Lipoproteins and Transfer to Milk Fat of Dietary (1-Carbon-14) Linoleic Acid in Lactating Cows, J. Dairy Sci. 61, 561–565.
- Lauryssens, M., Verbeke, R., and Peeters, G. (1961) Metabolism of Stearate-1-C14 in the Isolated Cow's Udder, J. Lipid Res. 2, 383–388.
- Emken, E.A., Adlof, R.O., Hachey, D.L., Garza, C., Thomas, M.R., and Brown-Booth, L. (1989) Incorporation of Deuterium-Labeled Fatty Acids into Human Milk Plasma, and Lipoprotein Phospholipids and Cholesteryl Esters, J. Lipid Res. 30, 395–402.
- Emken, E.A., Adlof, R.O., Duval, S.M., Shane, J.M., Waler, P. M., and Becker, C. (2004) Effect of Triacylglycerol Structure on Absorption and Metabolism of Isotope-Labeled Palmitic and Linoleic Acids by Humans. Lipids 39, 1–9. CrossRef
- Bosner, M.S., Ostlund, Jr, R.E., Osofisan O., Grosklos, J., Fritschle, C., and Lange, L.G. (1993) Assessment of Percent Cholesterol Absorption in Humans with Stable Isotopes. J. Lipid Res. 34, 1047–1053.
- Lin, Y.H., Pawlosky, R.J., and Salem, Jr, N. (2005) Simultaneous Quantitative Determination of Deuterium- and Carbon-13-Labeled Essential Fatty Acids in Rat Plasma. J. Lipid Res. 46, 1974–1982. CrossRef
- Differential biohydrogenation and isomerization of [U-13C]Oleic and [1-13C]Oleic acids by mixed ruminal microbes
Volume 41, Issue 5 , pp 513-517
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Department of Animal and Veterinary Science, University of Idaho, PO Box 442330, Moscow, 83844-2330, Moscow, Idaho
- 2. Dipartimento di Scienze Zootecniche, Università di Sassari, Via Enrico De Nicola, 9-07100, Sassari, Italy
- 3. Department of Animal Science, University of Padova, Viale dell'Università, 161-35020, Legnaro (PD), Italy
- 4. Department of Animal and Veterinary Sciences, Clemson University, 29634, Clemson, South Carolina