Lipids

, Volume 40, Issue 4, pp 329–334

Associations between apolipoprotein E genotype and circulating F2-isoprostane levels in humans

Authors

  • Marion Dietrich
    • School of Public HealthUniversity of California
  • Youqing Hu
    • School of Public HealthUniversity of California
  • Gladys Block
    • School of Public HealthUniversity of California
  • Estibaliz Olano
    • Hugh Sinclair Unit of Human Nutrition, School of Food BiosciencesUniversity of Reading
  • Lester Packer
    • Department of Molecular Pharmacology and Toxicology, School of PharmacyUniversity of Southern California
  • Jason D. Morrow
    • Division of Clinical PharmacologyVanderbilt University
  • Mark Hudes
    • Department of Nutritional SciencesUniversity of California
  • Gulbahar Abdukeyum
    • Hugh Sinclair Unit of Human Nutrition, School of Food BiosciencesUniversity of Reading
  • Gerald Rimbach
    • Institute of Human Nutrition and Food ScienceChristian Albrechts University
    • Hugh Sinclair Unit of Human Nutrition, School of Food BiosciencesUniversity of Reading
Articles

DOI: 10.1007/s11745-006-1390-4

Cite this article as:
Dietrich, M., Hu, Y., Block, G. et al. Lipids (2005) 40: 329. doi:10.1007/s11745-006-1390-4

Abstract

Apolipoprotein E (apoE), an important determinant of plasma lipoprotein metabolism, has three common alleles (ε2, ε3, and ε4). Population studies have shown that the risk of diseases characterized by oxidative damage, such as coronary heart disease and Alzheimer’s disease, is significantly higher in ɛ4 carriers. We evaluated the association between apoE genotypes and plasma F2-isoprostane levels, an index of lipid peroxidation, in humans. Two hundred seventy-four healthy subjects (104 males, 170 females; 46.9±13.0 yr; 200 whites, 74 blacks; 81 nonsmokers, 64 passive smokers, and 129 active smokers) recruited for a randomized clinical antioxidant intervention trial were included in this analysis. ApoE genotype was determined by PCR and restriction enzyme digestion. Free plasma F2-isoprostane was measured by GC-MS. Genotype groups were compared using multiple regression analysis with adjustment for sex, age, race, smoking status, body mass index, plasma ascorbic acid, and β-carotene. Subjects with ε3/ε4 and ε4/ε4 genotype (ε4-carriers) and with ε2/ε3 and ε3/ε3 (non-ε4-carriers) were pooled for analysis. In subjects with high cholesterol levels (total cholesterol above 200 mg/dl), plasma F2-isoprostane levels were 29% higher in ε4 carriers than in non-ε4-carriers (P=0.0056). High-cholesterol subjects that are ε4 carriers have significantly higher levels of lipid peroxidation as assessed by circulating F2-isoprostane levels.

Abbreviations

AD

Alzheimer’s disease

apoE

apolipoprotein E

BMI

body mass index

CHD

coronary heart disease

CM

chylomicrons

LDLC

LDL cholesterol

OR

odds ratio

Copyright information

© AOCS Press 2005