Lipids

, 40:807

Docosahexaenoic acid supplementation in vegetarians effectively increases omega-3 index: A randomized trial

  • Julia Geppert
  • Veronika Kraft
  • Hans Demmelmair
  • Berthold Koletzko
Articles

DOI: 10.1007/s11745-005-1442-9

Cite this article as:
Geppert, J., Kraft, V., Demmelmair, H. et al. Lipids (2005) 40: 807. doi:10.1007/s11745-005-1442-9

Abstract

Low red blood cell (RBC) membrane content of FPA+DHA (hereafter called omega-3 index) has recently been described as an indicator for increased risk of death from coronary heart disease. The relationship between plasma and RBC FA, focusing on omega-3 index, and the response to DHA supplementation were investigated in a double-blind, randomized, placebo-controlled, intervention study. Healthy vegetarians (87 f, 17 m) consumed daily a microalgae oil from Ulkenia sp. (0.94 g DHA/d) or olive oil (placebo) for 8 wk. DHA supplementation significantly increased DHA in RBC total lipids (7.9 vs. 4.4 wt%), in RBC PE (12.1 vs. 6.5 wt%), in RBC PC (3.8 vs. 1.4 wt%), and in plasma phospholipids (PL) (7.4 vs. 2.8 wt%), whereas EPA levels rose to a much lesser extent. Microalgae oil supplementation increased the omega-3 index from 4.8 to 8.4 wt%. After intervention, 69% of DHA-supplemented subjects (but no subject of the placebo group) reached an omega-3 index above the desirable value of 8 wt%. Omega-3 index and EPA+DHA levels in RBC PE, RBC PC, and plasma PL were closely correlated (r always >0.9). We conclude that an 8-wk supplementation with 0.94 g DHA/d from microalgae oil achieves a beneficial omega-3 index of ≥8% in most subjects with low basal EPA+DHA status. RBC total FA analyses can be used instead of RBC lipid fraction analyses for assessing essential FA status, e.g., in clinical studies.

Abbreviations

AA

arachidonic acid

ALA

α-linolenic acid

BMI

body mass index

CHD

coronary heart disease

DPA

docosapentaenoic acid

LA

linoleic acid

LCPUFA

long-chain polyunsaturated fatty acid

OA

oleic acid, 18∶1n−9

PA

palmitic acid, 16∶0

PL

phospholipids

RBC

red blood cells

SFA

saturated fatty acid

Copyright information

© AOCS Press 2005

Authors and Affiliations

  • Julia Geppert
    • 1
  • Veronika Kraft
    • 1
  • Hans Demmelmair
    • 1
  • Berthold Koletzko
    • 1
  1. 1.Div. Metabolic Diseases and Nutrition, Dr. von Hauner Children’s HospitalLudwig Maximilians University of MunichMunichGermany