Assessment of dietary and genetic factors influencing serum and adipose fatty acid composition in obese female identical twins
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Fourteen pairs of obese female monozygotic twins were recruited for a study of genetic influences on serum and adipose fatty acid (FA) composition. Following 1 wk of inpatient stabilization, fasting serum and adipose tissue obtained by surgical excision were analyzed by thin-layer and gas chromatography. Intrapair resemblances (IPR) for individual FA were assessed by Spearman rank correlation and by analysis of variance and were found in serum cholesteryl esters (CE), triglycerides (TG), and adipose TG. With two exceptions (CE linoleate and adipose eicosapentaenoate), these IPR were limited to the nonessential FA. Palmitate had significant IPR in four lipid fractions; in serum CE and adipose TG palmitate was strongly correlated with multiple measures of adiposity. In contrast to other lipid fractions, serum phosphatidylcholine (PC) FA had 12 IPR, of which 6 were essential FA including arachidonate (r=0.76, P<0.0005), eicosapentaenoate (r=0.78, P<0.0005), and docosahexaenoate (r=0.86, P<0.0001). The PC IPR could not be explained by analysis of preadmission 7-d food records. After dividing the pairs into two groups differing and nondiffering according to fat intake of individuals in the pair, there was no evidence of a gene-environment interaction between fat intake and FA composition. The IPR for nonessential FA indicate that there is active genetic control of either food choices or postabsorptive metabolic processing. The high level of IPR in the PC fraction in contrast to the other lipid fractions suggests strong genetic influence over selection of specific FA for this membrane fraction independent of diet.
- Raclot, T., and Groscolas, R. (1993) Differential Mobilization of White Adipose Tissue Fatty Acids According to Chain Length, Unsaturation, and Positional Isomerism, J. Lipid Res. 34, 1515–1526.
- Raclot, T., Mioskowski, E., Bach, A.C., and Groscolas, R. (1995) Selectivity of Fatty Acid Mobilization: A General Feature of Adipose Tissue, Am. J. Physiol. 296, R1060-R1067.
- Phinney, S.D., Tang, A.B., Johnson, S.B., and Holman, R.T. (1990) Reduced Adipose 18∶3 ω3 with Weight Loss by Very Low Calorie Dieting, Lipids 25, 798–806.
- Hudgins, L.C., and Hirsch, J. (1991) Changes in Abdominal and Gluteal Adipose Tissue Fatty Acid Compositions in Obese Subjects After Weight Gain and Weight Loss, Am. J. Clin. Nutr. 53, 1372–1377.
- Tang, A.B., Nishimura, K.Y., and Phinney, S.D. (1992) Preferential Reduction in Adipose Tissue α-Linolenic Acid(18∶3ω3) During Very Low Calorie Dieting Despite Supplementation with 18∶3ω3, Lipids 28, 987–993.
- Summers, L.K.M., Barnes, C., Fielding, B.A., Beysen, C., Ilie, V., Humpreys, S.M., and Fray, K.N. (2000) Uptake of Individual Fatty Acids into Adipose Tissue in Relation to Their Presence in the Diet, Am. J. Clin. Nutr. 71, 1470–1477.
- Odin, R.S., Finke, B.A., Blake, W.L., Phinney, S.D., and Clarke, S.D. (1987) Modification of Fatty Acid Composition of Membrane Phospholipid in Hepatocyte Monolayer with n−3, n−6, and n−9 Fatty Acids and Its Relationship to Triacylglycerol Production, Biochim. Biophys. Acta 921, 378–391.
- Clarke, S.D., and Jump, D.B. (1994) Dietary Polyunsaturated Fatty Acid Regulation of Gene Transcription, Annu. Rev. Nutr. 14, 83–98. CrossRef
- Pelikánová, T., Kouhout, M., Válek, J., Baše, J., and Kazdová, L. (1989) Insulin Secretion and Insulin Action Related to the Serum Phospholipid Fatty Acid Pattern in Healthy Men, Metabolism 38, 188–192. CrossRef
- Borkman, M., Storlien, L.H., Pan, D.A., Jenkins, A.B., Chisholm, D.J., and Campbell, L.V. (1993) The Relation Between Insulin Sensitivity and the Fatty Acid Composition on Skeletal Muscle Phospholipids, N. Engl. J. Med. 328, 238–244. CrossRef
- Baur, L.A., O'Connor, J., Pan, D.A., and Storlien, H. (1999) Relationship Between Maternal Risk of Insulin Resistance and the Child's Muscle Membrane Fatty Acid Composition, Diabetes 48, 112–116.
- Blond, J.P., Henchiri, C., and Bézard, J. (1989) Delta-6 and Delta-5 Desaturase Activities in Liver from Obese Zucker Rats at Different Ages, Lipids 24, 389–395.
- Guesnet, P., Bourre, J.-M., Guerre-Millo, M., Pascal, G., and Durand, G. (1990) Tissue Phospholipid Fatty Acid Composition in Genetically Lean or Obese Zucker Female Rats on the Same Diet, Lipids 25, 517–522.
- Phinney, S.D., Tang, A.B., Thurmond, D.C., Nakamura, M.T., and Stern, J.S. (1993) Abnormal Polyunsaturated Lipid Metabolism in the Obese Zucker Rat with Partial Metabolic Correction by γ-Linolenic Acid Administration, Metabolism 42, 1127–1140. CrossRef
- Phinney, S.D., Fisler, J.S., Tang, A.B., and Warden, C.H. (1994) Liver Fatty Acid Composition Correlates with Body Fat and Sex in a Multigenic Mouse Model of Obesity, Am. J. Clin. Nutr. 60, 61–67.
- Thurmond, D.C., Tang, A.B., Nakamura, M.T., Stern, J.S., and Phinney, S.D. (1993) Time-Dependent Effects of Progressive Gamma-Linoleate Feeding on Hyperphagia, Weight Gain, and Erythrocyte Fatty Acid Composition During Growth of Zucker Obese Rats, Obes. Res. 1, 118–125.
- Bouchard, C., Tremblay, A., Despres, J.-P., Nadeau, A., Lupien, P.J., Thériault, G., Dussault, J., Moorjani, S., Pinault, S., and Fournier, G. (1990) The Response to Long-Term Overfeeding in Identical Twins, N. Engl. J. Med. 322, 1477–1482. CrossRef
- Stunkard, A.J., Harris, J.R., Pedersen, N.L., and McClearn, G.E. (1990) The Body-Mass Index of Twins Who Have Been Reared Apart, N. Engl. J. Med. 322, 1483–1487. CrossRef
- Hainer, V., Stunkard, A.J., Kunesová, M., Pařízková, J., Štich, V., and Allison, D.B. (2000) Intrapair Resemblance in Very Low Calorie Diet Induces Weight Loss in Female Obese Identical Twins, Int. J. Obes. Relat. Metab. Disord. 24, 1051–1057. CrossRef
- Bates, C., Horrobin, D.F., and Ellis, K. (1992) Fatty Acids in Plasma Phospholipids and Cholesterol Esters from Identical Twins Concordant and Discordant for Schizophrenia, Schizophr. Res. 6, 1–7. CrossRef
- Stewart, M.E., McDonnell, M.W., and Downing, D.T. (1986) Possible Genetic Control of the Proportions of Branched-Chain Fatty Acids in Human Sebaceous Wax Esters, J. Invest. Dermatol. 86, 706–708. CrossRef
- Pařízková, J. (1977) Body Fat and Physical Fitness, 1st edn., pp. 32–51, M. Nijhoff, Hague.
- Brožek, J., Grande, F., Anderson, J.T., and Keys, A. (1967) Densitometric Analysis of Body Composition. Revision of Some Quantitative Assumptions, Am. N.Y. Acad. Sci. 110, 113–140.
- Meneely, G.R., and Kaltreider, N.L. (1949) The Volume of the Lung Determined by Helium Dilution: Description of the Method and Comparison with Other Procedures, J. Clin. Invest. 28, 129–139. CrossRef
- Durnin, J.V., and Wommersley, A.G. (1974) Body Fat Assessed from Total Body Density and Its Estimation from Skinfold Thickness: Measurements on 481 Men and Women Aged from 16 to 72 Years, Brit. J. Nutr. 32, 77–97. CrossRef
- Lohman, T., Roche, A., and Martorel, R., eds. (1989) Standardization of Anthropometric Measurements, pp. 39–80, Human Kinetics, Champaign.
- Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957) A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues, J. Biol. Chem. 226, 497–509.
- Carlson, L.A. (1985) Extraction of Lipids from Human Whole Serum and Lipoproteins and From Rat Liver Tissue with Methylene Chloride-Methanol: A Comparison with Extraction with Chloroform-Methanol, Clin. Chim. Acta 149, 89–93. CrossRef
- Hirsch, J. (1962) Composition of Adipose Tissue, in Adipose Tissue as an Organ (L.W. Kinsella, ed.), pp. 79–123, Thomas, Springfield, IL.
- Sinclair, H.M. (1982) The Relative Importance of Essential Fatty Acids of the Linoleic and Linolenic Families: Studies with an Eskimo Diet, Prog. Lipid. Res. 20, 897–899. CrossRef
- Phinney, S.D., Odin, R.S., Johnson, S.B., and Holman, R.T. (1990) Reduced Arachidonate in Serum Phospholipids and Cholesteryl Ester Associated with Vegetarian Diets in Humans, Am. J. Clin. Nutr. 51, 385–392.
- Björntorp, P. (1993) Visceral Obesity: A “Civilization Syndrome”, Obesity Res. 1, 206–222.
- Kissebah, A.H., and Krakower, G.R. (1994) Regional Adiposity and Morbidity, Physiol. Rev. 74, 761–811.
- Kunešová, M., Hainer, V., Hergetová, H., Žák, A., Pařízková, J., Hořejš, J., and Štich, V. (1995) Simple Anthropometric Measurements—Relation to Body Fat Mass, Visceral Adipose Tissue and Risk Factors of Atherogenesis, Sborn. Lék. 96, 257–268.
- Lands, W.E.M. (1995) Long-Term Fat Intake and Biomarkers, Am. J. Clin. Nutr. 61, 721S-725S.
- Hellerstein, M.K., Christiansen, M., Kaempfer, S., Kletke, C., Wu, K., Reid, J.S., Mulligan K., Hellerstein, N.S., and Shackleton, C.H. (1991) Measurement of de novo Hepatic Lipogenesis in Humans Using Stable Isotopes, J. Clin. Invest. 87, 1841–1852.
- Aarsland, A., Chinkes, D., and Wolfe, R.R. (1997) Hepatic and Whole-Body Fat Synthesis in Humans During Carbohydrate Overfeeding, Am. J. Clin. Nutr. 65, 1774–1782.
- Assessment of dietary and genetic factors influencing serum and adipose fatty acid composition in obese female identical twins
Volume 37, Issue 1 , pp 27-32
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Obesity Management Centre, Third Medical Department, First Medical School, Charles University, U nemocnice 1, 128 08, Prague, Czech Republic
- 2. Fourth Medical Department, Charles University, Prague
- 3. Galileo Laboratories, 95054, Santa Clara, California
- 4. Third Medical School, Charles University, Prague, Czech Republic
- 5. Department of Psychiatry, University of Pennsylvania, 19104, Philadelphia, Pennsylvania