Lipids

, Volume 36, Issue 4, pp 327–346

Atmospheric pressure chemical ionization mass spectrometry for analysis of lipids

Authors

    • Department of Chemistry & BiochemistryFlorida Atlantic University
Review

DOI: 10.1007/s11745-001-0725-5

Cite this article as:
Byrdwell, W.C. Lipids (2001) 36: 327. doi:10.1007/s11745-001-0725-5

Abstract

Atmospheric pressure chemical ionization (APCI) mass spectrometry (MS) has proven to be a very valuable technique for analysis of lipids from a variety of classes. This instrumental method readily produces useful ions with gentle fragmentation from large neutral molecules such as triacylglycerols and carotenoids, which are often difficult to analyze using other techniques. Molecules that are easily ionized, such as phospholipids, produce molecular ions and diagnostically useful fragment ions that are complementary to those produced by methods such as electrospray ionization MS with collision-induced dissociation. The simplicity and versatility of APCI-MS make it an ideal tool for use in solving hitherto very difficult analytical problems.

Abbreviations

ACN

acyl carbon number

AMVN

azobis(2,4-dimethylvaleronitrile)

APCI

atmospheric pressure chemical ionization

API

atmospheric pressure ionization

Br-MB

3-bromomethyl-7-methoxy-1,4-benzoxazin-2-one

CID

collision-induced dissociation

cSFC

capillary supercritical fluid chromatography

DAG

diacylglycerol

ECN

equivalent carbon number

EIC

extracted ion chromatogram

ELSD

evaporative light-scattering detector

ESI

electrospray ionization

FA

fatty acid

FAME

fatty acid methyl ester

FI

flow injection

FID

flame-ionization detection

GC

gas chromatography

L

linoleic acid

LC

liquid chromatography

Ln

linolenic acid

LSIMS

liquid secondary ion mass spectrometry

MAG

monoacylglycerol

MS

mass spectrometry

MS/MS

tandem mass spectrometry

O

oleic acid

P

palmitic acid

PC

phosphatidylcholine

PG

phosphatidylglycerol

RP-HPLC

reversed-phase high-performance liquid chromatography

S

stearic acid

SFC

supercritical fluid chromatography

SIM

selected ion monitoring

TAG

triacylglycerol

THCA

3α,7α,12α-trihydroxy-5β-cholestanic acid

TSP

thermospray

UV

ultraviolet

Vis

visible

Copyright information

© AOCS Press 2001