Article

Acta Physiologiae Plantarum

, Volume 21, Issue 4, pp 335-340

First online:

Effect of calcium restriction on cardenolide accumulation in two cell lines of Digitalis thapsi grown under different light regimes

  • Margarita CachoAffiliated withDepartamento de Fisiología Vegetal, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno Email author 
  • , Margarita MoránAffiliated withDepartamento de Fisiología Vegetal, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno
  • , Purificación CorcheteAffiliated withDepartamento de Fisiología Vegetal, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno
  • , Jorge Fernández-TárragoAffiliated withDepartamento de Fisiología Vegetal, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The effect of calcium-deprivation on growth and the production of cardenolides in two undifferentiated cell lines of Digitalis thapsi maintained under three different light regimes (16 h photoperiod, darkness, or continuous light) was investigated. Growth was stimulated by continuous light in both cell lines cultured in complete medium. The light regime did not affect cardenolide accumulation in the cells of the hypocotyl-derived line; by contrast, continuous light or darkness increased the production in the leaf-derived line. The elimination of calcium favoured cardenolide production independently of the origin of the suspensions and the light regime, this beneficial effect being predominantly manifested under continuous light.

Key words

Digitalis thapsi cardenolide suspension cultures cell lines light regimes calcium radioimmunoassay