Acta Physiologiae Plantarum

, Volume 35, Issue 4, pp 1363–1366

Comparison of ginsenoside composition in native roots and cultured callus cells of Panax quinquefolium L.

Short Communication

DOI: 10.1007/s11738-012-1151-3

Cite this article as:
Wang, J., Liu, H., Gao, WY. et al. Acta Physiol Plant (2013) 35: 1363. doi:10.1007/s11738-012-1151-3

Abstract

In order to compare the ginsenoside composition in native Panax quinquefolium and in suspension cultured cells derived from root callus, HPLC–ESI-MSn analysis was performed. Under the present HPLC–ESI-MSn conditions, ten ginsenosides from native root were acquired in the positive and negative ion modes, namely Rg1, Re, Ro, malonyl-Rb1, Rf, Rb1, Rc, Rb2, Rb3 and Rd. Only four ginsenosides (Rg1, Re, Rf and Rb1) were identified from callus cells. Radical scavenging activity of P. quinquefolium callus cells with 250 mg l−1 methanolic extract on 1,1-diphenyl-2-picrylhydrazyl (DPPH) was 55.72 %, while only 6.31 % DPPH inhibition was obtained in native root.

Keywords

Panax quinquefolium L.Suspension cellGinsenosideHPLC–ESI-MSnAntioxidant

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

KT

Kinetin

tR

Retention times

DPPH

1,1-Diphenyl-2-picrylhydrazyl

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Juan Wang
    • 1
    • 2
  • Hui Liu
    • 1
  • Wen-Yuan Gao
    • 1
    • 2
  • Liming Zhang
    • 1
  1. 1.Key Laboratory of Industrial Fermentation MicrobiologyMinistry of Education, Tianjin University of Science and TechnologyTianjinChina
  2. 2.Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and TechnologyTianjin UniversityTianjinChina