Comparison of ginsenoside composition in native roots and cultured callus cells of Panax quinquefolium L.
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In order to compare the ginsenoside composition in native Panax quinquefolium and in suspension cultured cells derived from root callus, HPLC–ESI-MSn analysis was performed. Under the present HPLC–ESI-MSn conditions, ten ginsenosides from native root were acquired in the positive and negative ion modes, namely Rg1, Re, Ro, malonyl-Rb1, Rf, Rb1, Rc, Rb2, Rb3 and Rd. Only four ginsenosides (Rg1, Re, Rf and Rb1) were identified from callus cells. Radical scavenging activity of P. quinquefolium callus cells with 250 mg l−1 methanolic extract on 1,1-diphenyl-2-picrylhydrazyl (DPPH) was 55.72 %, while only 6.31 % DPPH inhibition was obtained in native root.
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- Comparison of ginsenoside composition in native roots and cultured callus cells of Panax quinquefolium L.
Acta Physiologiae Plantarum
Volume 35, Issue 4 , pp 1363-1366
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- Panax quinquefolium L.
- Suspension cell
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- Author Affiliations
- 1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China
- 2. Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China