Acta Physiologiae Plantarum

, Volume 35, Issue 4, pp 1363–1366

Comparison of ginsenoside composition in native roots and cultured callus cells of Panax quinquefolium L.

Authors

  • Juan Wang
    • Key Laboratory of Industrial Fermentation MicrobiologyMinistry of Education, Tianjin University of Science and Technology
    • Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and TechnologyTianjin University
  • Hui Liu
    • Key Laboratory of Industrial Fermentation MicrobiologyMinistry of Education, Tianjin University of Science and Technology
    • Key Laboratory of Industrial Fermentation MicrobiologyMinistry of Education, Tianjin University of Science and Technology
    • Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and TechnologyTianjin University
  • Liming Zhang
    • Key Laboratory of Industrial Fermentation MicrobiologyMinistry of Education, Tianjin University of Science and Technology
Short Communication

DOI: 10.1007/s11738-012-1151-3

Cite this article as:
Wang, J., Liu, H., Gao, W. et al. Acta Physiol Plant (2013) 35: 1363. doi:10.1007/s11738-012-1151-3
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Abstract

In order to compare the ginsenoside composition in native Panax quinquefolium and in suspension cultured cells derived from root callus, HPLC–ESI-MSn analysis was performed. Under the present HPLC–ESI-MSn conditions, ten ginsenosides from native root were acquired in the positive and negative ion modes, namely Rg1, Re, Ro, malonyl-Rb1, Rf, Rb1, Rc, Rb2, Rb3 and Rd. Only four ginsenosides (Rg1, Re, Rf and Rb1) were identified from callus cells. Radical scavenging activity of P. quinquefolium callus cells with 250 mg l−1 methanolic extract on 1,1-diphenyl-2-picrylhydrazyl (DPPH) was 55.72 %, while only 6.31 % DPPH inhibition was obtained in native root.

Keywords

Panax quinquefolium L.Suspension cellGinsenosideHPLC–ESI-MSnAntioxidant

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

KT

Kinetin

tR

Retention times

DPPH

1,1-Diphenyl-2-picrylhydrazyl

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012