Short Communication

Acta Physiologiae Plantarum

, Volume 35, Issue 4, pp 1363-1366

First online:

Comparison of ginsenoside composition in native roots and cultured callus cells of Panax quinquefolium L.

  • Juan WangAffiliated withKey Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and TechnologyTianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University
  • , Hui LiuAffiliated withKey Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology
  • , Wen-Yuan GaoAffiliated withKey Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and TechnologyTianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University Email author 
  • , Liming ZhangAffiliated withKey Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology

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Abstract

In order to compare the ginsenoside composition in native Panax quinquefolium and in suspension cultured cells derived from root callus, HPLC–ESI-MSn analysis was performed. Under the present HPLC–ESI-MSn conditions, ten ginsenosides from native root were acquired in the positive and negative ion modes, namely Rg1, Re, Ro, malonyl-Rb1, Rf, Rb1, Rc, Rb2, Rb3 and Rd. Only four ginsenosides (Rg1, Re, Rf and Rb1) were identified from callus cells. Radical scavenging activity of P. quinquefolium callus cells with 250 mg l−1 methanolic extract on 1,1-diphenyl-2-picrylhydrazyl (DPPH) was 55.72 %, while only 6.31 % DPPH inhibition was obtained in native root.

Keywords

Panax quinquefolium L. Suspension cell Ginsenoside HPLC–ESI-MSn Antioxidant