Original Paper

Acta Physiologiae Plantarum

, Volume 35, Issue 3, pp 801-810

Open Access This content is freely available online to anyone, anywhere at any time.

The role of PnACO1 in light- and IAA-regulated flower inhibition in Pharbitis nil

  • Emilia WilmowiczAffiliated withChair of Plant Physiology and Biotechnology, Nicolaus Copernicus University
  • , Kamil FrankowskiAffiliated withChair of Plant Physiology and Biotechnology, Nicolaus Copernicus University Email author 
  • , Jacek KęsyAffiliated withChair of Plant Physiology and Biotechnology, Nicolaus Copernicus University
  • , Paulina GlazińskaAffiliated withChair of Plant Physiology and Biotechnology, Nicolaus Copernicus University
  • , Waldemar WojciechowskiAffiliated withChair of Plant Physiology and Biotechnology, Nicolaus Copernicus University
  • , Agata KućkoAffiliated withChair of Plant Physiology and Biotechnology, Nicolaus Copernicus University
  • , Jan KopcewiczAffiliated withChair of Plant Physiology and Biotechnology, Nicolaus Copernicus University

Abstract

In this study, the first ACC oxidase (PnACO1) cDNA from model short-day plant Pharbitis nil was isolated. The expression pattern of PnACO1 was studied under different conditions (photoperiod and auxin), an adequate balance of which determines P. nil flowering. It was shown that the gene was transcribed in all the examined organs of the 5-day-old seedling and was strongly activated by auxin. Our results also revealed that PnACO1 transcript accumulation in the cotyledons showed diurnal oscillations under both LD and SD conditions. On the basis of presented and previously obtained data, we suggest that flowering inhibition evoked by IAA in P. nil results from its stimulatory effect on both ACC synthase and oxidase gene expression and, consequently, enhances ethylene production.

Keywords

ACC oxidase Auxin Ethylene Flowering Pharbitis nil (Ipomoea nil)