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The effect of endogenous hydrogen peroxide induced by cold treatment in the improvement of tissue regeneration efficiency

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Abstract

We propose that oxidative stress resulting from an imbalance between generation and scavenging hydrogen peroxide contributes to tissue regeneration efficiency during somatic embryogenesis of hexaploid winter wheat (Triticum aestivum cv. Kamila) and organogenesis of faba bean (Vicia faba ssp. minor cv. Nadwislanski). Endogenous hydrogen peroxide content and antioxidant capacity of cells were determined in initial explants and callus cultures derived from these explants. Regeneration-competent explants (immature embryos) contained more endogenous H2O2 than explants initiated from regeneration-recalcitrant tissue (mature wheat embryos and faba bean epicotyls). Higher H2O2 levels were observed despite the higher activity of antioxidative enzymes (superoxide dismutase and catalase) and the induction of their gene expression. Calli originating from immature embryos retained the capacity of the initial explants: high H2O2 production was observed during the whole culture period. Low temperature treatment (4°C) was found to be an effective factor, which improved both regeneration ability and H2O2 production. Exogenous application to the medium of H2O2 and catalase blocker (3-aminotriazole), but not FeEDTA and superoxide dismutase blocker (diethyldithiocarbamate), also resulted in the enhancement of regeneration efficiency. These results clearly indicate that plant regeneration is specifically regulated by endogenous H2O2 and by factors, which improve its accumulation. Moreover, a study of the activity of various SOD isoforms suggests that not only the absolute concentration of H2O2, but also its localisation might be responsible for controlling regeneration processes.

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Abbreviations

CAT:

Catalase [EC 1.11.1.6]

NR:

Regeneration-recalcitrant tissue

R:

Tissue potentially competent to regenerate, or regenerating tissue

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase [EC 1.15.1.1.]

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Authors and Affiliations

  1. Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland

    Magdalena Szechyńska-Hebda, Edyta Skrzypek & Maria Wędzony

  2. Institute of Genetics and Molecular Biology, Nicolas Copernicus University, Gagarina 9, 87-100, Toruń, Poland

    Grażyna Dąbrowska

  3. Pedagogical University of Krakow, Podchorążych 2, 30-084, Kraków, Poland

    Maria Wędzony

  4. Laboratory of Plant Cell Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Andre van Lammeren

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  1. Magdalena Szechyńska-Hebda
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Correspondence to Magdalena Szechyńska-Hebda.

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Szechyńska-Hebda, M., Skrzypek, E., Dąbrowska, G. et al. The effect of endogenous hydrogen peroxide induced by cold treatment in the improvement of tissue regeneration efficiency. Acta Physiol Plant 34, 547–560 (2012). https://doi.org/10.1007/s11738-011-0852-3

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