Acta Physiologiae Plantarum

, Volume 33, Issue 4, pp 1467–1474

Proteomic identification of differentially expressed proteins in mature and germinated maize pollen

  • Yunhao Zhu
  • Pengfei Zhao
  • Xiaolin Wu
  • Wei Wang
  • Monica Scali
  • Mauro Cresti
Original Paper

DOI: 10.1007/s11738-010-0683-7

Cite this article as:
Zhu, Y., Zhao, P., Wu, X. et al. Acta Physiol Plant (2011) 33: 1467. doi:10.1007/s11738-010-0683-7

Abstract

The identification of proteins involved in pollen germination and tube growth is important for fundamental studies of fertility and reproduction in flowering plants. We used 2-DE and MALDI-TOF-MS to identify differentially expressed proteins in mature (P0) and 1-h germinated (P1) maize pollen. Among about 470 proteins separated in 2D gels, the abundances of 26 protein spots changed (up- or down-regulation) between P0 and P1. The 13 up-regulated protein spots were mainly involved in tube wall modification, actin cytoskeleton organization, energy metabolism, signaling, protein folding and degradation. In particular, pectin methylesterase, inorganic pyrophosphatase, glucose-1-phosphate uridylyltransferase and rab GDP dissociation inhibitor α are highly up-regulated, suggesting their potential role in pollen tube growth. The down-regulated 13 protein spots mainly include defense-related proteins, pollen allergens and some metabolic enzymes. This study would contribute to the understanding of the changes in protein expression associated with pollen tube development.

Keywords

Mass spectrometryPollen germinationProteomeTwo-dimensional gel electrophoresisZea mays

Abbreviations

2D

Two dimensional

2-DE

Two-dimensional electrophoresis

ADK

Adenosine kinase

MPP

Mitochondrial-processing peptidase

MALDI-TOF

Matrix-assisted laser desorption/ionization time-of-flight

Mr

Molecular weight

MS

Mass spectrometry

P0

Mature pollen

P1

Pollen germinated for 1 h

pI

Isoelectric point

PMEs

Pectin methylesterases

rab-GDI

rab GDP dissociation inhibitor

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  • Yunhao Zhu
    • 1
  • Pengfei Zhao
    • 1
  • Xiaolin Wu
    • 1
  • Wei Wang
    • 1
    • 2
  • Monica Scali
    • 3
  • Mauro Cresti
    • 3
  1. 1.College of Life ScienceHenan Agricultural UniversityZhengzhouChina
  2. 2.Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan ProvinceZhengzhouChina
  3. 3.Dipartimento di Scienze Ambientali ‘G. Sarfatti’Università di SienaSienaItaly