Acta Physiologiae Plantarum

, Volume 31, Issue 5, pp 995–1002

Effects of feeding Clostera anachoreta on hydrogen peroxide accumulation and activities of peroxidase, catalase, and ascorbate peroxidase in Populus simonii × P. pyramidalis ‘Opera 8277’ leaves

Authors

  • Zeng-Hui Hu
    • Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants (Ministry of Education), College of Biological Sciences and TechnologyBeijing Forestry University
    • Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants (Ministry of Education), College of Biological Sciences and TechnologyBeijing Forestry University
  • Fan-Yi Shen
    • College of SciencesBeijing Forestry University
  • Xiao-Hua Su
    • Research Institute of ForestryChinese Academy of Forestry
Original Paper

DOI: 10.1007/s11738-009-0316-1

Cite this article as:
Hu, Z., Shen, Y., Shen, F. et al. Acta Physiol Plant (2009) 31: 995. doi:10.1007/s11738-009-0316-1

Abstract

In response to Clostera anachoreta larvae attack, poplar (Populus simonii × P. pyramidalis ‘Opera 8277’) leaves produced a high level of hydrogen peroxide (H2O2). Histochemical localization revealed that H2O2 was mainly localized in herbivore-wounded zones and might spread through the veins. The activities of three H2O2-scavenging enzymes, i.e., peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT), were also enhanced in herbivore-wounded leaves, and exhibited an opposite pattern to the accumulation of H2O2. It was found that diphenylene iodonium chloride (DPI, a special inhibitor of NADPH oxidase) treatment significantly inhibited the accumulation of H2O2 induced by herbivory damage. Moreover, DPI treatment led to an obvious decrease in the activities of POD, APX, and CAT. The results indicated that NADPH oxidase contributed to the accumulation of H2O2 and the increase in activities of H2O2-scavenging enzymes in poplar leaves induced by herbivory damage. The balance between H2O2-production pathway and H2O2-scavenging enzymes led to the tolerable level of H2O2 acting in P. simonii × P. pyramidalis ‘Opera 8277’ cuttings in response to herbivory damage.

Keywords

Activities of H2O2-scavenging enzymesClostera anachoretaHydrogen peroxidePopulus simonii × P. pyramidalis ‘Opera 8277’

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2009