Acta Physiologiae Plantarum

, Volume 31, Issue 4, pp 693–698

An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

  • Shashi Kant Singh
  • Manoj K. Rai
  • Pooja Asthana
  • L. Sahoo
Original Paper

DOI: 10.1007/s11738-009-0280-9

Cite this article as:
Singh, S.K., Rai, M.K., Asthana, P. et al. Acta Physiol Plant (2009) 31: 693. doi:10.1007/s11738-009-0280-9


An efficient and improved in vitro propagation system for Spilanthes acmella L. using transverse thin cell layer (tTCL) culture system was established. The frequency of shoot regeneration from tTCL nodal segments was affected by concentrations of plant growth regulators and orientation of the explant. MS (Murashige and Skoog in Physiol Plant 15:473–497, 1962) medium with 5.0 mg dm−3 BAP was optimal for shoot regeneration. Upon this medium, the explant inoculated in the upright orientation exhibited a high frequency of shoot regeneration (about 97%), and the highest number of shoots (31.5) per explant. The intact node (1.0–1.5 cm) cultured on the same medium had significantly lower shoot multiplication ability with only 4.5 shoots per responsive explant. As compared to BAP alone, the combination of BAP and Kin or NAA did not have positive effects on shoot multiplication from tTCL nodal segments. Rooting of shoots was achieved on growth regulator free full-strength MS medium. Plantlets were transplanted into soil with 90–100% survival rate.


In vitro propagation Organogenesis Plant growth regulators Spilanthes acmella Thin cell layer explant 







Murashige and Skoog (1962)


α-Naphthalene acetic acid


Plant growth regulators


Transverse thin cell layer

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2009

Authors and Affiliations

  • Shashi Kant Singh
    • 1
    • 2
  • Manoj K. Rai
    • 3
  • Pooja Asthana
    • 3
  • L. Sahoo
    • 2
  1. 1.Department of Life Sciences, Institute of Plant BiologyNational Taiwan UniversityTaipeiTaiwan
  2. 2.Center for EnergyIndian Institute of Technology GuwahatiGuwahatiIndia
  3. 3.Laboratory of Morphogenesis, Department of BotanyBanaras Hindu UniversityVaranasiIndia