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Temperature-dependent impact of 24-epibrassinolide on the fatty acid composition and sugar content in winter oilseed rape callus

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Abstract

The aim of this experiment was to study the effect of 24-epibrassinolide (BR27) on fatty acids composition and sugar content in winter oilseed rape callus cultured at 20 and 5°C. Studies have showed that BR27 action is highly temperature-dependent. The increase in sugar content (sucrose, glucose and fructose) by BR27 in concentration 100 nM was observed only in calli cultured at 20°C. At 5°C, quite the opposite effect of BR27 action was observed; where cold increased the sugar content, BR27 decreased it. BR27 at 20°C had a similar effect on the fatty acid composition of phospholipids (PL) as the cold in the process of frost hardening of oilseed rape calli. BR27 decreased the 16:0, 18:1 and 18:2 and increased the 18:3 fatty acid content. At 5°C, BR27 (100 nM) generally did not influence the fatty acid composition of PL. In case of digalactosyl diacylglycerols and monogalactosyl diacylglycerols, the influence of BR27 on the fatty acid composition is ambiguous but still depends on temperature.

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Abbreviations

PL:

Phospholipids

DG:

Digalactosyl diacylglycerols

MG:

Monogalactosyl diacylglycerols

BR27 :

24-Epibrassinolide (according to Mandava 1988; Zullo and Kohout 2004)

BR:

Brassinosteroids

16:0:

Palmitic acid

18:0:

Stearic acid

18:1:

Oleic acid

18:2:

Linoleic acid

18:3:

Linolenic acid

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Authors and Affiliations

  1. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 21 Niezapominajek Street, 30-239, Kraków, Poland

    Anna Janeczko, Andrzej Skoczowski, Iwona Idzik, Jolanta Biesaga-Kościelniak & Elżbieta Niemczyk

  2. Department of Plant Physiology, Faculty of Agriculture, Agricultural University of Kraków, 3 Podłużna Street, 30-239, Kraków, Poland

    Katarzyna Hura

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  1. Anna Janeczko
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  2. Katarzyna Hura
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Correspondence to Anna Janeczko.

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Communicated by S. Lewak.

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Janeczko, A., Hura, K., Skoczowski, A. et al. Temperature-dependent impact of 24-epibrassinolide on the fatty acid composition and sugar content in winter oilseed rape callus. Acta Physiol Plant 31, 71–79 (2009). https://doi.org/10.1007/s11738-008-0202-2

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  • DOI: https://doi.org/10.1007/s11738-008-0202-2

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