Acta Physiologiae Plantarum

, Volume 30, Issue 5, pp 581–593

Betalain production in plant in vitro systems

  • Vasil Georgiev
  • Mladenka Ilieva
  • Thomas Bley
  • Atanas Pavlov
Review

DOI: 10.1007/s11738-008-0170-6

Cite this article as:
Georgiev, V., Ilieva, M., Bley, T. et al. Acta Physiol Plant (2008) 30: 581. doi:10.1007/s11738-008-0170-6

Abstract

Betalains have been widely used as natural colorants for many centuries, but their attractiveness for use as colorants of foods (or drugs and cosmetics) has increased recently due to their reportedly high anti-oxidative, free radical scavenging activities and concerns about the use of various synthetic alternatives. The main commercial sources of betalains are powders and concentrates of red beet (Beta vulgaris) or cactus pear (Opuntia ficus-indica) extracts. However, in recent years the technical and commercial feasibility of various in vitro systems to produce them biotechnologically has been explored. These research activities have included assessments of novel approaches for cultivating plant cell or tissue cultures, and diverse bioreactor systems for increasing production levels of secondary metabolites. This paper reviews recent progress in plant in vitro systems for producing betalain pigments. In addition, the factors that could be manipulated, the bioreactor systems that could be used, and the strategies that could be applied to improve betalain production are discussed.

Keywords

BetalainsBiological activityBioreactorsPlant in vitro systemsProcess optimization

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

B5

Gamborg’s B5 medium

BAP

6-Benzylaminopurine

DOPA

3,4-dihydroxy-l-phenylalanine

DPPH

1,1-Diphenyl-2-picrylhydrazyl

LDL

Low density lipoproteins

MS

Murashige and Skoog medium

ROS

Reactive oxygen species

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2008

Authors and Affiliations

  • Vasil Georgiev
    • 1
  • Mladenka Ilieva
    • 1
  • Thomas Bley
    • 2
  • Atanas Pavlov
    • 1
  1. 1.Department of Microbial Biosynthesis and Biotechnologies, Laboratory in PlovdivThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of SciencesPlovdivBulgaria
  2. 2.Institute of Food Technology and Bioprocess EngineeringDresden University of TechnologyDresdenGermany