Acta Physiologiae Plantarum

, Volume 30, Issue 2, pp 183–199

Identification of dehydration responsive genes from two non-nodulated alfalfa cultivars using Medicago truncatula microarrays

  • Dong Chen
  • Ming-Xiang Liang
  • Daryll DeWald
  • Bart Weimer
  • Michael D. Peel
  • Bruce Bugbee
  • Jacob Michaelson
  • Elizabeth Davis
  • Yajun Wu
Original Paper

DOI: 10.1007/s11738-007-0107-5

Cite this article as:
Chen, D., Liang, MX., DeWald, D. et al. Acta Physiol Plant (2008) 30: 183. doi:10.1007/s11738-007-0107-5

Abstract

To have a comprehensive understanding of how legume plants respond to drought at the gene expression level and examine whether legume plants that are not fixing nitrogen would behave similar to non-legume plants in drought response, transcriptomes were studied in two non-nodulated alfalfa (Medicago sativa L.) cultivars, Ladak and 53V08, when plants were subjected to dehydration stress. Two heat shock-related protein genes were up-regulated in the 3-h stressed shoots in both cultivars. One of them was also up-regulated in the 8-h stressed shoots, along with dehydrin and LEA. A xyloglucan endotransglycosylase and a gene with unknown function were down-regulated in both 3- and 8-h stressed shoots. In roots, nearly half of the 55 genes commonly up-regulated at 3 h are involved in pathogen resistance, insect defense and flavonoid synthesis, which differs from other dehydration-responsive transcriptomes in the literature. Many known drought-responsive genes, such as LEA and dehydrin, were up-regulated after 8 h of treatment. The genes encoding caffeoyl-CoA O-methyl transferase and dirigent were up-regulated in the 3-h stressed roots, while two aquaporin genes were down-regulated, suggesting that lignification and prevention of water loss in roots in initial dehydration stress is a common strategy for both cultivars. The results also indicate the involvement of some specific signal transduction pathways, osmotic adjustment and ion homeostasis regulation during dehydration response. Besides those known dehydration-responsive genes in the literature, some dehydration responses and genes in alfalfa appear to be unique. Our results provide valuable insight into a comprehensive understanding of dehydration response in alfalfa at the molecular level.

Keywords

Dehydration stressAlfalfaMicroarrayGene expression

Supplementary material

11738_2007_107_MOESM1_ESM.doc (477 kb)
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11738_2007_107_MOESM2_ESM.doc (298 kb)
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11738_2007_107_MOESM4_ESM.doc (2.4 mb)
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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2007

Authors and Affiliations

  • Dong Chen
    • 1
  • Ming-Xiang Liang
    • 1
    • 2
  • Daryll DeWald
    • 1
    • 3
  • Bart Weimer
    • 1
    • 4
  • Michael D. Peel
    • 5
  • Bruce Bugbee
    • 2
  • Jacob Michaelson
    • 1
  • Elizabeth Davis
    • 2
  • Yajun Wu
    • 2
  1. 1.Center for Integrated BioSystemsUtah State UniversityLoganUSA
  2. 2.Department of Plants, Soils, and ClimateUtah State UniversityLoganUSA
  3. 3.Department of BiologyUtah State UniversityLoganUSA
  4. 4.Department of Nutrition and Food SciencesUtah State UniversityLoganUSA
  5. 5.USDA-ARS Forage and Range Research LaboratoryUtah State UniversityLoganUSA