Skip to main content
Log in

In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

An efficient, rapid and reproducible plant regeneration protocol was successfully developed for Cassia angustifolia using nodal explants excised from 14-day-old aseptic seedlings. Of the two cytokinins, 6-benzyladenine (BA) and thidiazuron (TDZ) evaluated as supplements to Murashige and Skoog (MS) medium, TDZ at an optimal concentration of 5.0 μM was effective in inducing multiple shoots. The highest rate of shoot multiplication was achieved on MS medium supplemented with 5.0 μM TDZ and 1.0 μM indole-3-acetic acid (IAA) at pH 5.8. The regenerated shoots when subcultured on hormone free MS medium considerably increased the rate of shoot multiplication and shoot length by end of fourth subculture passage. Rooting was achieved on the isolated shoots using MS medium with 60 μM indole -3- butyric acid (IBA) and 1% activated charcoal for 1 week and subsequently transferring the shootlets to half strength MS liquid media without IBA and activated charcoal. The in vitro raised plantlets with well-developed shoot and roots were successfully established in earthen pots containing garden soil and grown in greenhouse.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

BA:

6-Benzyladenine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog medium

NAA:

α-Naphthalene acetic acid

TDZ:

Thidiazuron

References

  • Agrawal V, Sardar PR (2003) In vitro organogenesis and histomorphological investigations in Senna (Cassia angustifolia)—a medicinally valuable shrub. Physiol Mol Biol Plants 9:131–140

    Google Scholar 

  • Agrawal V, Sardar PR (2006) In vitro propagation of Cassia angustifolia through leaflet and cotyledon derived calli. Biol Plant 50:118–122

    Article  CAS  Google Scholar 

  • Ajithkumar D, Seeni S (1998) Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree. Plant Cell Rep 17:422–426

    Article  CAS  Google Scholar 

  • Anis M, Husain MK, Shahzad A (2005) In vitro plantlet regeneration of Pterocarpus marsupium Roxb., an endangered leguminous tree. Curr Sci 88:861–863

    CAS  Google Scholar 

  • Anonymous (1992) The wealth of India: a dictionary of Indian raw materials and industrial products, vol 3. CSIR, New Delhi, pp 354–363

  • Caboni ED, Angeli S, Chiappetta A, Innocenti AM, Van Onckelen H, Damiano C (2002) Adventitious shoot regeneration from vegetative shoot apices in pear and putative role of cytokinin accumulation in the morphogenetic process. Plant Cell Tissue Organ Cult 70:199–206

    Article  CAS  Google Scholar 

  • Faisal M, Anis M (2006) Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia. Biol Plant 50:437–440

    Article  CAS  Google Scholar 

  • Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tissue Organ Cult 80:187–190

    Article  CAS  Google Scholar 

  • Feyissa T, Welander M, Negash L (2005) Micropropagation of Hagenia abyssinica (Bruce) J.F. Gmel: a multipurpose tree. Plant Cell Tissue Organ Cult 80:119–127

    Article  Google Scholar 

  • Franz G (1993) The senna drug and its chemistry. Pharmocology 47:2–6

    CAS  Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119

    Article  CAS  Google Scholar 

  • Kanyand M, Peterson CM (1997) The differentiation of emergences into adventitious shoots in peanut, Arachis hypogaea (L.). Plant Sci 126:87–95

    Article  CAS  Google Scholar 

  • Koroch K, Juliani HP, Kapteyn J, Simon JE (2003) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tissue Organ Cult 69:79–83

    Article  Google Scholar 

  • Malik KA, Saxena PK (1992) Regeneration in Phaseolus vulgaris L. high frequency induction of direct shoot formation in intact seedlings by N6-benzyl aminopurine and thidiazuron. Planta 186:384–389

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murthy BNS, Victor J, Singh RPS, Fletcher RA, Saxena PK (1996) In vitro regeneration of chickpea (Cicer arietinum L.): stimulation of direct organogenesis and somatic embryogenesis by thidiazuron. Plant Growth Regul 19:233–240

    Article  CAS  Google Scholar 

  • Parliman BJ, Evans PT, Rupert EA (1982) Tissue culture of single rhizome explants of Dionaea muscipula Ellis ex. L., the venus fly trap for rapid asexual propagation. HortScience 107:305–310

    CAS  Google Scholar 

  • Pence VC (1999) The application of biotechnology for the conservation of endangered plants. In: Benson EE (ed) Plant conservation biotechnology, vol 15. Taylor & Francis, London, pp 227–241

  • Shailendra V, Joshi N, Tak K, Purohit SD (2005) In vitro adventitious shoot bud differentiation and plantlet regeneration in Feronia limonia L. (swingle). In Vitro Cell Dev Biol Plant 41:296–302

    Article  Google Scholar 

  • Tiwari V, Tiwari KN, Singh BD (2001) Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tissue Organ Cult 66:9–16

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. Anis.

Additional information

Communicated by E. Lojkowska.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Siddique, I., Anis, M. In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant. Acta Physiol Plant 29, 233–238 (2007). https://doi.org/10.1007/s11738-007-0029-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-007-0029-2

Keywords

Navigation