The effect of drought stress on chlorophyll fluorescence in Lolium-Festuca hybrids
Received: 20 June 2005 Accepted: 29 November 2005 DOI:
Cite this article as: cielniak, J.K., Filek, W. & cielniak, J.BK. Acta Physiol Plant (2006) 28: 149. doi:10.1007/s11738-006-0041-y Abstract
The effects of drought on photochemical efficiency of PSII in leaves of 22 hybrids of
Festuca pratensis × Lolium multiflorum and Festuca pratensis × Lolium perenne and of Festuca pratensis cv. Skra were investigated. A significant decrease of electron transport efficiency (about 25%) in PSII (Φ PSII) was not found before 9 days of seedling growth in hydroponics with water potential (Ψ w) equal to −0.8 MPa (simulated “soil drought”). The decrease of Φ PSII was similarly related to that of excitation energy capture by open PSII reaction centre (Fv’/Fm’) and also to the decrease of the proportion of oxidized to reduced Q A (photochemical fluorescence quenching, q p). According to the drought prolongation, variation of all parameters of fluorescence between genotypes significantly increased. The seedlings of some genotypes were able to recover electron transport efficiency in PSII after increasing water potential in nutrient solution (removing the “soil drought”).
When plants grew in containers with soil and 4 genotypes with the highest sensitivity of electron transport to drought (S) as well as 4 genotypes with the highest tolerance (T) were compared 17 days after watering ceased, Ψ
w in leaves considerably decreased, but the differences between S and T genotypes were often not significant in this respect. The differences between S and T genotypes, as values of Fv/Fm were concerned, also appeared small (about 5%), similarly as that of Fv’/Fm’ (5%), q p (12%) and Φ PSII (about 15%).
Drought stress increased non-photochemical quenching of chlorophyll fluorescence (NPQ) 15 to 47% and this could protect the PSII reaction centres from damages because of energy excess. The increase of NPQ was not closely connected with drought resistance of plants because it was similar in some genotypes tolerant to dehydration as well as in sensitive ones.
The results of the experiments suggest that resources of genetic variability in
Festulolium may be sufficient for revealing differences between genotypes on the basis of measurement of chlorophyll a fluorescence, as far as their tolerance to soil drought is concerned. As the tolerance of PSII against drought is high, the determinations of fluorescence should be performed rather under severe stress. Such methods seem to be useful for selection of genotypes with high drought tolerance as well as with the ability to at least partial repairing of PSII after drought. Key words breeding drought tolerance chlorophyll a fluorescence Festuca pratensis Lolium multiflorum Lolium perenne Festulolium List of abbreviations S, T
sensitive and tolerant
Festulolium hybrids, respectively Φ PSII
quantum efficiency of PSII electron transport
photochemical fluorescence quenching
efficiency of excitation energy capture by open PSII reaction centres
efficiency of excitation capture by open PSII in dark-adapted leaves
non-photochemical quenching of chlorophyll fluorescence
the primary stable electron acceptor of PSII
References Akmal M., Janssens M.J.J. 2004
. Productivity and light use efficiency of perennial ryegrass with contrasting water and nitrogen supplies. Field Crops Res. 88: 143–155.
CrossRef Google Scholar Araus J.L., Amaro T., Voltas J., Nakkoul H., Nachit M.M. 1998
. Chlorophyll fluorescence as a criterion for grain yield in durum wheat under Mediterranean conditions. Field Crops Res. 55: 209–223.
CrossRef Google Scholar Bártak M., Nijs I., Impes I. 1996
. The effect of long-term exposure of
L. plants to elevated CO
and /or air temperature on quantum yield of photosystem 2 and net photosynthesis. Photosynthetica, 32(4): 549–562.
Google Scholar Bártak, M., Nijs I., Impes I. 1998
. The susceptibility of PSII of
to sudden fall in air temperature-response of plants grown in elevated CO
and /or air temperature. Envir. Exp. Bot. 39: 85–95.
CrossRef Google Scholar Benveniste-Levkovitz P., Canaani O., Gromet-Elhanan Z., Atsmon D. 1993
. Characterization of drought resistance in a wild relative of wheat,
. Photosynth. Res. 35: 149–158.
CrossRef Google Scholar Bresti M., Cornic G., Fryer M.J., Baker N.R. 1995
. Does photorespiration protect the photosynthetic apparatus in French bean leaves from photoinhibition during drought stress? Planta 196: 450–457.
Google Scholar Colom M.R., Vazzana C. 2003
. Photosynthesis and PSII functionality of drought-resistant and drought-sensitive weeping lovegrass plants. Envir. Exp. Bot. 49: 135–144.
CrossRef Google Scholar Cornic G., Briantais J.M. 1991
. Partitioning of photosynthetic electron flow between CO
reduction in a C
L.) at different CO
concentrations and during drought stress. Planta 183: 178–184.
CrossRef Google Scholar Cornic G. 1994
. Drought stress and high light effects on leaf photosynthesis. In: Baker N.B., Bowyer J.R. (eds). Photoinhibition of photosynthesis: from molecular mechanisms to the field. Oxford, UK, Bios Scientific Publishers 297–313.
Google Scholar Dib T.L., Monneveux Ph., Acevedo E., Nachit M.M. 1994
. Evaluation of proline analysis and chlorophyll fluorescence quenching measurements as drought tolerance indicators in durum wheat (
). Euphytica 79: 65–73.
CrossRef Google Scholar Durand J.-L. Gastal F., Etchebest S., Bonnet A.-C., Ghesquière M. 1997
. Interspecific variability of plant water status and leaf morphogenesis in temperate forage grasses under summer water deficit. Europ. J. Agron. 7: 99–107.
CrossRef Google Scholar Eickmeier W.G., Casper C., Osmond C.B. 1993
. Chlorophyll fluorescence in the resurrection plant
and evidence for zeaxanthin-associated photoprotection. Planta 189: 30–38.
CrossRef Google Scholar Erez M., Lannoye R. 1991. Quantification of physiological disorders in stressed plants. p. 414–433. In: E. Acevedo, A.P. Conesa, P. Monneveux, J.P. Srivastava (Eds). Physiology-Breeding of Winter Cereals for Stresses Mediterranean Environments. Montpellier, France, 3–6 July 1989. Colloques 55. Flagella Z., Campanile R.G., Ronga G., Stopelli M.C., Pastore D., De Caro A., Di Fonzo N. 1996
. The maintenance of photosynthetic electron transport in relation to osmotic adjustment in durum wheat cultivars differing in drought resistance. Plant Sci. 118: 127–133
CrossRef Google Scholar Flagella Z., Pastore D., Campanile R.G., Di Fonzo N. 1994
. Photochemical quenching of chlorophyll fluorescence and drought tolerance in different durum wheat (
) cultivars. J. Agr. Sci. 122: 183–192.
Google Scholar Flagella Z., Pastore D., Campanille R.G., Di Fonzo N. 1995
. The quantum yield of photosynthetic electron transport evaluated by chlorophyll fluorescence is a probe of drought tolerance in durum wheat. J. Agric. Sci. Cambridge 125: 325–329.
Google Scholar Flexas J., Briantais J-M., Cerovic Z., Medrano H., Moya I. 2000
. Steady-state and maximum chlorophyll fluorescence responses to water stress in grapevine leaves: a new remote sensing system. Remote Sens. Environ. 73: 283–297.
CrossRef Google Scholar Franca A., Loi A., Davies W.J. 1998
. Selection annual ryegrass for adaptation to semi-arid condition. Eur. J. Agron. 9: 71–78.
CrossRef Google Scholar Genty B., Briantais J-M., Baker N.R. 1989
. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta 990: 87–92.
Google Scholar Ghesquière M., Emilie J.-C., Jadas-Hècard J., Mousset C., Traineau R., Poisson C. 1996
. First in
assessment of feeding value of
hybrids derived from
and selection for palatability. Plant Breed. 115: 238–244.
CrossRef Google Scholar Golding A.J., Johnson G.N. 2003
. Down-regulation of linear and activation of cyclic electron transport during drought. Planta 218: 107–114.
PubMed CrossRef Google Scholar Haupt-Herting S, Fock H.P. 2002
. Oxygen exchange in relation to carbon assimilation in water-stressed leaves during photosynthesis. Ann. Bot. 89: 851–859.
PubMed CrossRef Google Scholar Havaux M. 1992
. Stress tolerance of photosystem II
. Plant Physiol. 100: 424–432.
PubMed CrossRef Google Scholar Havaux M., Ernez M., Lannoye R. 1988
. Correlation between heat tolerance and drought tolerance in cereals demonstrated by rapid chlorophyll fluorescence tests. J. Plant Physiol. 133: 555–560.
Google Scholar Havaux M., Lannoye R. 1985
chlorophyll fluorescence and delayed light emission as rapid technique for stress tolerance in crop plants. Z. Pflanzenzüchtung 95: 1–13.
Google Scholar Hill J., Michaelson-Yates T.P.T. 1987
. Effect of competition upon the productivity of white clover perennial ryegrass mixture: analysis of the interrelations between characters. Plant Breed. 98: 161–170.
CrossRef Google Scholar Hoagland, D.R. Arnon, D.I. 1938. The water-culture method for growing plants without soil. Univ.Calif.Exp. Sta. Cir. 347. Huang B., Fry J., Wang B. 1998
. Water relations and canopy characteristics of tall fescue cultivars during and after drought stress. Hort. Science 33 (5): 837–840.
Google Scholar Huang B., Gao H. 2000
. Root physiological characteristics associated with drought resistance in tall fescue cultivars. Crop Sci. 40: 196–203.
CrossRef Google Scholar Humhreys M., Thomas H. 1993
. Improved drought resistance in introgression lines derived from
Lolium multiglorum x Festuca arundinacea
hybrids. Plant Breed. 111: 155–161.
CrossRef Google Scholar Krause G.H., Weis F. 1991
. Chlorophyll fluorescence and photosynthesis: the basic. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42: 313–349.
CrossRef Google Scholar Lichtenthaler H.K. 1988
chlorophyll fluorescence as a tool for stress detection in plants. In: Applications of Chlorophyll Fluorescence. H.K. Lichtenthaler (Ed). Dordrecht, The Netherlands, Kluwer Acad. Publ.: 129–142.
Google Scholar Loggini B., Scartazza A., Brunoli E., Navari-Izzo F. 1999
. Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol. 119: 1091–1099.
PubMed CrossRef Google Scholar Lu C., Zhang J. 1998
. Effects of water stress on photosynthesis, chlorophyll fluorescence and photoinhibition in wheat plants. Aust. J. Plant Physiol. 25: 883–892.
CrossRef Google Scholar Lu C., Zhang J. 1999
. Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants. J. Exp. Bot. 50: 1199–1206.
CrossRef Google Scholar Nelson C.J., Asay K.H., Sleper D.A. 1977
. Mechanisms of canopy development of tall fescue genotypes. Crop Sci. 17: 449–452.
CrossRef Google Scholar Nijs I., Teughels H., Blum H., Hendrey G., Impens I. 1996
. Simulation of climate change with infrared heaters reduces the productivity of
L. in summer. Envir. Exp. Bot. 36 (5): 271–280.
CrossRef Google Scholar Onillon B., Durand J.-L., Gastal F., Tournebize R. 1995
. Drought effects on growth and carbon partitioning in a tall fescue sward grown at two rates of nitrogen fertilization. Eur. J. Agron. 4: 91–99.
Google Scholar Osmond C.D. 1994
. What is photoinhibition? Some insights from comparison of sun and shade plants. In: Baker N.R., Bowyer J.R. (eds.) Photoinhibition of photosynthesis. From molecular mechanisms to the field. Bios, Oxford: 1–24.
Google Scholar Ourcival J.M., Methy M., Burgess R. 1992
. Chlorophyll fluorescence analysis of genotypic variability of drought stress response of white clover (
) and perennial rye (
). Can. J. Bot. 70: 1556–1562.
Google Scholar Schreiber U., Bigler W. 1987
. Rapid assessment of stress effects on plant leaves by chlorophyll fluorescence measurements. In: Plant Response to Stress. J.D. Tenhunen, F.M. Catarino, O.L. Lange, C. Oechel (Eds). Berlin, Springer-Verlag: 27–53,
Google Scholar Smirnoff N. 1993
. The role of active oxygen in response of plants to water deficit and desiccation. New. Phytol. 125: 27–58.
CrossRef Google Scholar Tourneux C., Peltier G. 1995
. Effect of water deficit on photosynthetic oxygen measuring using
and mass spectrometry in
L. leaf disc. Planta 195: 570–577.
CrossRef Google Scholar van Rensburg L., Krüger G.H.J. 1993
. Differential inhibition of photosynthesis (
vitro), and changes chlorophyll a fluorescence induction kinetics of four tobacco cultivars under drought stress. J. Plant Physiol. 141: 357–365.
Google Scholar White R.H., Engelke M.C., Morton S.J., Ruemmele B.A. 1992
. Competitive turgor maintenance in tall fescue. Crop Sci. 32: 251–256.
CrossRef Google Scholar Zwierzykowski Z., Tayyar R., Brunell M., Lukaszewski A.J. 1998
. Genome recombination in intergeneric hybrids between tetraploid
. J. Heredity 89 (4): 324–328.
CrossRef Google Scholar Zwierzykowski Z., Zwierzykowska E., Kosmala A., Łuczak M., Jok W. 2003. Genome recombination in early generations of Festuca pratensis × Lolium perenne hybrids. In: Z. Zwierzykowski, M. Surma and P. Kachlicki (eds.). Application of Novel Cytogenetic and Molecular Techniques. Institute of Plant Genetics, Polish Academy of Sciences Pozna : 63–69. Copyright information
© Department of Plant Physiology 2009