Antioxidative defense system in lupin roots exposed to increasing concentrations of lead
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Yellow lupin (Lupinus luteus) cv. Juno seedlings exposed to increasing concentrations of Pb2+ (50–350 mg l−1) were analysed in respect to its effect on the degradation of lipids, the content of antioxidants (ascorbate, α-tocopherol) and the activity of the ascorbate glutathione cycle enzymes (dehydroascorbate reductase DAR; EC 18.104.22.168 and glutathione reductase GR; EC 22.214.171.124). Lipid peroxidation, expressed as the content of TBArm (thiobarbituric acid reactive metabolites), increased only at 50 and 100 mg l−1 Pb2+, whereas at higher lead concentrations it decreased as compared with the control. The level of free fatty acids was not significantly affected as a result of Pb2+ exposure, except for 300 mg l−1 Pb2+. The content of α-tocopherol increased significantly at the range of concentrations between 150 and 300 mg l−1 and at these concentrations lipid peroxidation was inhibited. Ascorbic acid (AA) and dehydroascorbic acid (DHA) content increased considerably in roots treated with 100 and 150 mg l−1 Pb2+. In general the activity of DHAR and GR was stimulated by lead, however at higher Pb2+ concentrations (300 and 350 mg l−1) GR revealed lower enzymatic activity. Our results suggest that in lead-treated roots lipids might be protected against reactive oxygen species (ROS) by lipid-soluble molecules of α-tocopherol and this direct defense seems to be supported by AA as well as the enzymes: DHAR and GR.
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- Antioxidative defense system in lupin roots exposed to increasing concentrations of lead
Acta Physiologiae Plantarum
Volume 28, Issue 4 , pp 357-364
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- dehydroascorbate reductase
- glutathione reductase
- Industry Sectors
- Author Affiliations
- 1. Laboratory of Plant Ecophysiology, Adam Mickiewicz University, Faculty of Biology, A1. Niepodległo ci 14, PL-61-713, Pozna, Poland
- 2. Institute of Dendrology, Polish Academy of Sciences, ul. Parkowa 5, PL-62-035, Kórnik, Poland