Changes in the composition of fatty acids and sterols of membrane lipids during induction and differentiation of Brassica napus (var. oleifera L.) callus
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Changes in the membrane lipid and sterols content and composition were studied during induction and differentiation in callus cultures of Brassica napus var. oleifera. Callus induction was associated with an increase of DGDG content, significant changes in fatty acids composition of all lipid fractions and increased degree of lipid unsaturation. The membrane lipid composition of tissue at different degrees of differentiation was found to vary significantly, particularly two weeks after transfer of callus to regeneration medium. The main differences concerned the content and composition of galactolipids. Curiously in many cases, these differences declined during subsequent culture, in spite of the morphogenesis process which was in progress. Another result of differentiation was the change in free sterol composition: in shoot regenerating calli the content of stigmasterol had rose whereas the accumulation of campesterol decreased. Even though observed changes in membrane properties may not play a role in morphogenesis they are nevertheless useful as developmental markers and can be invaluable in understanding biochemical basis of morphogenesis.
- Bhardwaj L., Merillon J.-M., Ramawat K. G. 1995. Changes in the composition of membrane lipids in relation to differentiation in Aegle marmelos callus cultures. Plant Cell, Tissue Organ Culture 42: 33–37. CrossRef
- Bligh E.G., Dyer W. J. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.
- Brenac P., Sauvaire Y. 1996. Accumulation of sterols and steroidal sapogenins in developing fenugreek pods: possible biosynthesis in situ. Phytochemistry 41: 415–422. CrossRef
- Caldwell C.L., Whitman C.E. 1987. Temperature-induced protein conformational changes in barley root plasma membrane-enriched microsomes. Plant Physiol. 84: 918–923. CrossRef
- Cunha A., Ferreira M.F. 1997. Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax. Plant Science 124: 97–105. CrossRef
- Douce R., Joyard J. 1980. Plant galactolipids. In: The Biochemistry of Plants, Vol.4, Lipids: Structure and Function, ed. by P. K. Stumpf, Academic Press, New York: 321–362.
- Duxbury C. L., Legge R. L., Paliyath G., Barber R. F., Thompson J. E. 1991. Alternations in membrane protein conformation in response to senescence-related changes. Phytochemistry 30: 63–68. CrossRef
- Flosh J., Lees M., Sloaney-Stanley G. H. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226: 497–509.
- Grunwald C. 1975. Plant sterols. Ann. Rev. Plant Physiol. 26: 209–236. CrossRef
- Hartmann M. A., Normand G., Benveniste P. 1975. Sterol composition of plasma membrane enriched-fractions from maize coleoptiles. Plant Sci. Lett. 5: 287–292. CrossRef
- Kuiper P. J. C. 1985. Environmental changes and lipid metabolism of higher plants. Physiol. Plant. 64: 118–122. CrossRef
- Manoharan K., Prasad R., Guha-Mukherjee S. 1987. Greening and shoot-differentiation related lipid changes in callus cultures of Datura innoxia. Phytochemistry 26: 407–410. CrossRef
- Mills G. L., Lane P. A., Weech P. K. 1989. A guidebook to lipoprotein technique. R.H. Burdon, P.H. Van Knippenberg, Elsevier Sci. Pub., Amsterdam, New York, Oxford.
- Moore T. S. 1982. Phospholipid biosynthesis. Ann. Rev. Plant Physiol. 33: 235–259. CrossRef
- Murashige T., Skoog F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497. CrossRef
- Rivera C. M., Penner D. 1978. Rapid changes in soybean root membrane lipids with altered temperature. Phytochemistry 17: 1269–1272. CrossRef
- Ryyppö A., Vapaavuori E. M., Rikala R., Sutinen M.-L. 1994. Fatty acid composition of microsomal phospholipids and H+-ATPase activity in the roots of Scots pine seedlings grown at different root temperatures during flushing. J. Exp. Bot. 45: 1533–1539. CrossRef
- Tattrie N. H., Veliky I. A. 1973. Fatty acid composition of lipids in various plant cell cultures. Can. J. Bot. 51: 513–516.
- Van Blitterswijk W. J., van Hoeven R. P., van der Meer B. W. 1981. Lipid structural order parameters (reciprocal fluidity) in biomembranes derived from steady-state fluorescence polarization measurements. Biochim. Biophys. Acta 644: 323–332. CrossRef
- Williams M., Francis D., Hann A. C., Harwood J. L. 1991. Changes in lipid composition during callus differentiation in cultures of oilseed rape (Brassica napus L.). J. Exp. Bot. 42: 1551–1556. CrossRef
- Wilson K. J., Stillwell W., Maxam T., Baldrige T. 1991. Membrane fluidity changes in embryogenic and non-embryogenic cultures of Asclepias and Daucus in response to auxin removal. Physiol. Plant. 82: 633–639. CrossRef
- Yoshida S., Uemura M. 1984. Protein and lipid composition of isolated plasma membranes from orchard grass (Dactylis glomerata L.) and changes during cold acclimation. Plant Physiol. 75: 31–37.
- Changes in the composition of fatty acids and sterols of membrane lipids during induction and differentiation of Brassica napus (var. oleifera L.) callus
Acta Physiologiae Plantarum
Volume 24, Issue 1 , pp 3-10
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Brassica napus
- fatty acid composition
- oilseed rape
- regeneration ability
- Industry Sectors