, Volume 9, Issue 1, pp 13-16
Date: 01 Feb 2011

Effects of herb-partitioned moxibustion and electroacupuncture on serum indexes of intestinal fibrosis in rats with Crohn’s disease

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Abstract

Objective

To observe the impact of herb-partitioned moxibustion and electroacupuncture on hyaluronic acid (HA), procollagen III (PC III) and collagen IV (C IV) of intestinal fibrosis in rats with Crohn’s disease (CD), and discuss the effect and the mechanism of acupuncture on treating intestinal fibrosis of rats.

Methods

SD rats were randomly divided into control group, model group, herb-partitioned moxibustion group, electroacupuncture group and medicine group. The model of CD was developed by trinitrobenzene sulfonic acid. Masson stain was used to observe the collagen fibers hyperplasia in colon and radioimmunoassay was adopted to detect the content of HA, PC III and C IV in serum.

Results

Compared with the control group, the proliferation of colonic collagen fibers, as well as the HA, PC III and C IV in serum increased in the model group. Compared with the model group, the expression of collagen fibers, serum HA, PC III and C IV decreased in the herb-partitioned moxibustion group, electroacupuncture group and medicine group. The expression of collagen fibers serum HA, PC III and C IV in the herb-partitioned moxibustion group were lower than in the electroacupuncture group and medicine group.

Conclusion

Herb-partitioned moxibustion and electroacupuncture effectively improved the pathological state of intestinal fibrosis in rats with CD and reduced the content of HA, PC III and C IV in serum.

摘要

目的

观察隔药灸和电针对克罗恩病(Crohn’s Disease, CD) 肠纤维化大鼠血清透明质酸 (Hyaluronic Acid, HA)、 III型前胶原(Procollagen III, PC III)、 IV型胶原(Collagen IV, C IV)的影响, 探讨针灸抗CD大鼠肠纤维化的作用与机制。

方法

SD大鼠随机分为正常组、 模型组、 隔药灸组、 电针组和药物组。 采用三硝基苯磺酸制备大鼠CD模型, 应用Masson染色光镜下观察结肠胶原纤维增生情况, 采用放射免疫分析技术检测大鼠血清HA、 PC III、 C IV含量。

结果

与正常组比较, 模型组大鼠结肠组织胶原纤维增生, 血清HA、 PC III、 C IV含量增加; 与模型组比较, 隔药灸组、 电针组及西药组胶原纤维表达减少, 血清中HA、 PC III、 C IV含量降低, 与电针组、 西药组比较, 隔药灸组胶原纤维表达减少, 血清CIV 含量降低。

结论

隔药灸和电针均可改善CD大鼠肠纤维化病理状态, 降低血清HA、 PC III、 C IV含量。

Fund Items: National Basic Research Program of China (973 Program, 2009CB522900); National Natural Sciences Funds (30973785); Shanghai Leading Academic Discipline Project (S30304)
Author: Ren Yanbo, master of medicine