, Volume 9, Issue 1, pp 1-6
Date: 01 Feb 2011

Acupuncture on the basic fibroblast growth factor and type I collagen in colons of rats with Crohn’s disease

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Abstract

Objective

To observe the impacts of herb-partitioned moxibustion, warm moxibustion and electroacupuncture on the basic fibroblast growth factor (bFGF) and collagen type I (Col I) in colons of rats with Crohn’ disease (CD), and discuss the mechanism of acupuncture therapy on the intestinal fibrosis in CD.

Methods

The model rats were developed by TNBS as multiple proinflammatory method. The rats were randomly divided into 5 groups: a normal group, a model group, a warm moxibustion group, an electroacupuncture group and a herb-partitioned moxibustion group. The treatments were carried out at Tianshu (ST 25) (bilateral) and Qihai (CV 6) in different treatments. The immunohistochemistry was used to detect the expression position of Col I and bFGF.

Results

The expressions of Col I and bFGF in colons of rats in the model group significantly increased (compared with the normal group, P<0.01). After the herb-partitioned moxibustion, warm moxibustion and electroacupuncture, the expressions of Col I and bFGF reduced markedly in the rats with CD (P<0.01). The expression of bFGF and Col Iin the colons had an obvious correlation in the Spearman rank correlation analysis.

Conclusion

Acupuncture treatment reduced the abnormally high levels of expressions for Col I and bFGF in colons. Col I and bFGF participated in the fibrosis. Acupuncture treatment may reduce the bFGF expression in colons to regulate the excessive deposition, treating the intestinal fibrosis in CD.

摘要

目的

观察隔药灸、 温和灸、 电针对克罗恩病 (Crohn’s Disease, CD)大鼠结肠碱性成纤维细胞生长因子(Basic Fibroblast Growth Factor, bFGF)、 I型胶原, (Collagen Type I, ColI)表达的调控作用, 探讨针灸治疗克罗恩病肠壁纤维化的作用机制。

方法

采用TNBS多次致炎方法制备大鼠克罗恩病模型。 将大鼠随机分为正常组、 模型组、 温和灸组、 电针组和隔药灸组。 各针灸治疗组选取天枢、 气海穴分别采用不同的针灸方法进行治疗。 采用免疫组织化学法观察大鼠结肠组织ColI、 bFGF蛋白的分布和表达。

结果

模型组大鼠结肠组织Col I、 bFGF蛋白表达显著增强(与正常组相比, P<0.01); 经过隔药灸、 温和灸、 电针治疗后, CD大鼠Col I、 bFGF蛋白表达明显减少(P<0.01)。

结肠组织bFGF与Col I蛋白Spearman 等级相关分析结果表明, 两者具有明显相关性(P<0.01)。 结论: 针灸可以下调CD大鼠结肠异常增高的 Col I、 bFGF蛋白表达。 bFGFCol I蛋白参与了大鼠CD肠纤维化过程, 针灸可能通过降低结肠bFGF 蛋白表达, 从而调节Col I蛋白的沉积而发挥对CD肠纤维化的治疗作用。

Fund Items: National Basic Research Program of China (973 Program, 2009CB522900); National Natural Science Foundation of China (30973785); The Key Laboratory of Acupuncture-immune Effects of State Administration of Traditional Chinese Medicine