Acupuncture on the basic fibroblast growth factor and type I collagen in colons of rats with Crohn’s disease
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To observe the impacts of herb-partitioned moxibustion, warm moxibustion and electroacupuncture on the basic fibroblast growth factor (bFGF) and collagen type I (Col I) in colons of rats with Crohn’ disease (CD), and discuss the mechanism of acupuncture therapy on the intestinal fibrosis in CD.
The model rats were developed by TNBS as multiple proinflammatory method. The rats were randomly divided into 5 groups: a normal group, a model group, a warm moxibustion group, an electroacupuncture group and a herb-partitioned moxibustion group. The treatments were carried out at Tianshu (ST 25) (bilateral) and Qihai (CV 6) in different treatments. The immunohistochemistry was used to detect the expression position of Col I and bFGF.
The expressions of Col I and bFGF in colons of rats in the model group significantly increased (compared with the normal group, P<0.01). After the herb-partitioned moxibustion, warm moxibustion and electroacupuncture, the expressions of Col I and bFGF reduced markedly in the rats with CD (P<0.01). The expression of bFGF and Col Iin the colons had an obvious correlation in the Spearman rank correlation analysis.
Acupuncture treatment reduced the abnormally high levels of expressions for Col I and bFGF in colons. Col I and bFGF participated in the fibrosis. Acupuncture treatment may reduce the bFGF expression in colons to regulate the excessive deposition, treating the intestinal fibrosis in CD.
采用TNBS多次致炎方法制备大鼠克罗恩病模型。 将大鼠随机分为正常组、 模型组、 温和灸组、 电针组和隔药灸组。 各针灸治疗组选取天枢、 气海穴分别采用不同的针灸方法进行治疗。 采用免疫组织化学法观察大鼠结肠组织ColI、 bFGF蛋白的分布和表达。
模型组大鼠结肠组织Col I、 bFGF蛋白表达显著增强(与正常组相比, P<0.01); 经过隔药灸、 温和灸、 电针治疗后, CD大鼠Col I、 bFGF蛋白表达明显减少(P<0.01)。
- Yu WH, Xu GQ. Mechanism of fibrosis and the change of serum fibrostic markers in Crohn’s disease. International Journal of Digestive Diseases 2009, 29(4): 235–238.
- Wu F, Chakravarti S. Differential expression of inflammatory and fibrogenic genes and their regulation by NF-kappaB inhibition in a mouse model of chronic colitis. J Immunol, 2007, 179(10): 6988–7000.
- Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, Wallace JL. Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology, 1989, 96(3): 795–803.
- Theiss AL, Fruchtman S, Lund PK. Growth factors in inflammatory bowel disease: The actions and interactions of growth hormone and insulin-like growth factor-I. Inflamm Bowel Dis, 2004, 10(6): 871–880. CrossRef
- Koutroubakis IE, Petinaki E, Dimoulios P, Vardas E, Roussomoustakaki M, Maniatis AN, Kouroumalis EA. Serum laminin and collagen IV in inflammatory bowel disease. J Clin Pathol, 2003, 56(11): 817–820. CrossRef
- Pucilowska JB, Williams KL, Lund PK, Fibrogenesis IV. Fibrosis and inflammatory bowel disease: cellular mediators and animal models. Am J Physiol Gastrointest Liver Physiol, 2000, 279(4): G653–G659.
- Vallance BA, Gunawan MI, Hewlett B, Bercik P, Van Kampen C, Galeazzi F, Sime PJ, Gauldie J, Collins SM. TGF-beta1 gene transfer to the mouse colon leads to intestinal fibrosis. Am J Physiol Gastrointest Liver Physiol, 2005, 289(1): G116–G128. CrossRef
- Burke JP, Mulsow JJ, O’Keane C, Docherty NG, Watson RW, O’Connell PR. Fibrogenesis in Crohn’s disease. Am J Gastroenterol, 2007, 102(2): 439–448. CrossRef
- Yan JC, Chen WB, Ma Y, Ding TL. Pathological significance of bFGF/bFGF mRNA expression in liver tissues with chronic hepatitis B. Chinese Journal of Infectious Diseases, 2004, 22(5): 314–317.
- Chen WB, Yan JC, Ma Y, et al. Expression of basic fibroblast growth factor in hepatic tissues of hepatitis B. Chinese Journal of Clinical and Experimental Pathology, 2002, 18(5): 527–530.
- Xiang JJ, Sun JY, Deng N, et al. Involvement of bFGF in the lung response to silica in a mouse model. Chinese Journal of Pathophysiology, 2004, 20(4): 647–650.
- Iwata A, Masago A, Yamada K. Expression of bFGF mRNA after transient focal ischemia: comparision with expression of c-fos,c-jun, and hsp70 mRNA. J Neuro Trauma, 1997, 14(4): 201–210.
- Gass P, Herdegen T, Bravo R, Kiessling M. Induction of immediate early gene encoded proteins in the rat hippocampus after bicuculline-induced seizures: differential expression of KROX-24, Fos and Jun proteins. Neuroscience, 1992, 48(2): 315–324. CrossRef
- Ruggiero DA, Sica AL, Anwar M, Frasier I, Gootman N, Gootman PM. Induction of c-fos gene expression by spinal cord transection in Sus scrofa. Brain Res, 1997, 759(2): 301–305. CrossRef
- Del-Bel EA, Borges CA, Defino HL, Guimarães FS. Induction of Fos protein immunore activity by spinal cord contusion. Braz J Med Biol Res, 2000, 33(5): 521–528. CrossRef
- Rosenfeld H, Lee DJ, Grinnell F. Increased c-fos mRNA expression by human fibroblasts contracting stressed collagen matrices. Mol Cell Biol, 1998, 18(5): 2659–2667.
- Xu Q, Liu Y, Gorospe M, Udelsman R, Holbrook NJ. Acute hypertension activates mitogen-activated protein kinases in arterial wall. J Clin Invest, 1996, 97(2): 508–514. CrossRef
- Mu JQ, Wei LQ. Intervention function of bfgf antibody on model of pulmonary fibrosis. Zhong Guo Ying Yong Sheng Li Xue Za Zhi, 2010, 26(2): 222–226.
- Acupuncture on the basic fibroblast growth factor and type I collagen in colons of rats with Crohn’s disease
Journal of Acupuncture and Tuina Science
Volume 9, Issue 1 , pp 1-6
- Cover Date
- Print ISSN
- Online ISSN
- Shanghai Research Institute of Acupuncture and Meridian
- Additional Links
- Acupuncture-moxibustion Therapy
- Crohn Disease
- Fibroblast Growth Factor, Basic
- Collagen Type I
- 成纤维细胞生长因子, 碱性
- Author Affiliations
- 1. Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, P. R. China
- 2. Shanghai Research Institute of Acupuncture and Moxibustion, Shanghai, 200030, P. R. China
- 3. Shanghai Research Institute of Qigong, Shanghai, 200030, P. R. China