Regulating effects of different moxibustion therapies on bax and Bcl-2 in rats with ulcerative colitis
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(1)各治疗组结肠大体形态损伤积分和光镜下组织学损伤积分较模型组比较有明显改善(P<0.05, P<0.01); (2)UC模型大鼠结肠组织Bcl-2 mRNA的表达显著高于正常大鼠(P <0.01), 而Bax mRNA的表达显著低于正常大鼠(P<0.01); (3)治疗后, 隔药灸组、 隔姜灸组、 隔蒜灸组大鼠结肠组织Bcl-2 mRNA的表达明显下调, 与模型组比较有显著差异(P<0.05 或 P<0.01);隔药灸组、 隔姜灸组Bax mRNA的表达明显增高, 与模型组比较有显著差异(P<0.05)。
初步研究结果提示隔药灸、 隔姜灸、 隔蒜灸、 温和灸治疗均能有效改善UC大鼠结肠病理损伤, 隔药灸、 隔姜灸、 隔蒜灸可不同程度抑制UC大鼠结肠组织Bcl-2 mRNA和Bax mRNA的表达, 调节Bcl-2/Bax mRNA的比例关系。
Rats model of UC was made with immune methods and local stimulation. The models were treated with different moxibustion therapies 14 times, and then the expression of Bcl-2 and Bax mRNA were tested in the colonic tissue with fluorescent quantitative PCR.
(1)General scores of colon and histologic scores of injury were improved in all treatment groups except the model group (P<0.05, P<0.01); (2) The expression of Bcl-2 mRNA in the model rats of UC was higher than that in the normal rats (P<0.01), whereas Bax lower (P<0.01); (3) After treatments, the expression of Bcl-2 mRNA was lower in the herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion groups than that in the model group (P<0.05, P<0.01), and Bax mRNA in the herb cake-partitioned and ginger-partitioned groups was higher (P<0.05).
Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, garlic-partitioned moxibustion, and mild moxibustion could improve the pathological injury in UC rats. Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion could inhibit the expression of Bcl-2 mRNA and Bax mRNA in the colonic tissue of UC rats to regulate the ratio of Bcl-2/Bax mRNA.
- Regulating effects of different moxibustion therapies on bax and Bcl-2 in rats with ulcerative colitis
Journal of Acupuncture and Tuina Science
Volume 8, Issue 3 , pp 174-180
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- Shanghai Research Institute of Acupuncture and Meridian
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- 结肠炎, 溃疡性
- 基因, bcl-2
- Moxibustion Therapy
- Colitis, Ulcerative
- Genes, bcl-2
- Gene Expression