Obesity Surgery

, Volume 26, Issue 12, pp 3001–3006

Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism

  • Laura Hernández Hurtado
  • Francisco M. Sánchez-Margallo
  • José Luis De la Cruz Vigo
  • Juan Maestre Antequera
  • Ana M. Matos Azevedo
  • Javier G. Casado
  • Idoia Díaz-Güemes Martín-Portugués
Original Contributions

DOI: 10.1007/s11695-016-2223-z

Cite this article as:
Hernández Hurtado, L., Sánchez-Margallo, F.M., De la Cruz Vigo, J.L. et al. OBES SURG (2016) 26: 3001. doi:10.1007/s11695-016-2223-z

Abstract

Background

The objective of the study was to assess Roux-en-Y independent-to-weight metabolic effects on an obese Gottingen minipig.

Methods

Eleven castrated Göttingen minipigs were enrolled. Minipigs became obese after a 9-month period of experimental diet. The animals were then subjected to a laparoscopic Roux-en-Y gastric bypass (period P1) followed by 4 months of follow-up (period P2). During this last period, they were fed to maintain similar body weight to that observed at the day of surgery. Registered parameters during each period included biometric variables; abdominal adipose tissue (AT) distribution; lipid profile; HOMA-IR; and fasting plasma leptin, adiponectin, glucose, insulin and fructosamine.

Results

Two pigs were unable to complete the study due to postoperative complications. Four months after surgery, the body weight (kg) was maintained (P1 51.90 ± 5.78 vs P2 51.47 ± 5.68). On the contrary, intraperitoneal AT (p < 0.0001), glucose (mmol/L) (P1: 5.53 ± 0.37 vs 4.66 ± 0.72, p = 0.019), fructosamine (μmol/L) (p = 0.0001) and insulin (pmol/L) (P1: 36.66 ± 13.94 vs P2: 21.42 ± 12.62, p = 0.001) significantly decreased after surgery. Lipid profile showed a significant increase after high-fat feeding period and a slight reduction at P2, which continued increasing. At the end of the study, leptin plasma levels (ng/mL) showed a significant reduction compared to basal value (p = 0.001).

Conclusions

The improvement in glucose metabolism seems to occur by means of the redistribution of body fat. In order to fully confirm this hypothesis, more studies including the evaluation of incretins and inflammatory molecules, as well as a glycaemia control study against glucose load, are needed.

Keywords

Göttingen minipig Leptin Adiponectin Adipose tissue distribution 

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Laura Hernández Hurtado
    • 1
  • Francisco M. Sánchez-Margallo
    • 1
  • José Luis De la Cruz Vigo
    • 2
  • Juan Maestre Antequera
    • 1
  • Ana M. Matos Azevedo
    • 1
  • Javier G. Casado
    • 1
  • Idoia Díaz-Güemes Martín-Portugués
    • 1
  1. 1.Minimally Invasive Surgery Centre Jesús UsónCáceresSpain
  2. 2.Clínica San FranciscoLeónSpain