Obesity Surgery

, Volume 24, Issue 12, pp 2161–2168

Laparoscopic Adjustable Gastric Banding Reduces Subcutaneous Adipose Tissue and Blood Inflammation in Nondiabetic Morbidly Obese Individuals

  • Laura Iaffaldano
  • Carmela Nardelli
  • Vincenzo Pilone
  • Giuseppe Labruna
  • Andreina Alfieri
  • Donatella Montanaro
  • Maddalena Ferrigno
  • Maria Restituta Zeccolella
  • Nicola Carlomagno
  • Andrea Renda
  • Alfonso Baldi
  • Pietro Forestieri
  • Lucia Sacchetti
  • Pasqualina Buono
Original Contributions

DOI: 10.1007/s11695-014-1326-7

Cite this article as:
Iaffaldano, L., Nardelli, C., Pilone, V. et al. OBES SURG (2014) 24: 2161. doi:10.1007/s11695-014-1326-7

Abstract

Background

Significant and sustained excess weight loss (EWL) appears to reduce the risk of obesity-related comorbidities (insulin resistance, hyperlipidemia, and inflammation), but this has been primarily shown in adult diabetic obese patients. We evaluated whether the EWL obtained 3 years after laparoscopic adjustable gastric banding (LAGB) improves the metabolic phenotype in nondiabetic morbidly obese (NDMO) individuals from south Italy.

Methods

Serum and subcutaneous adipose tissue (SAT) samples from 20 obese individuals (median BMI = 41.5 kg/m2) before (T0) and after LAGB (T1) and from 10 controls (median BMI = 22.8 kg/m2) were taken. Serum leptin, adiponectin, C reactive protein (CRP), and main analyte levels were evaluated by routine methods or immunoassay. In SAT, adipocyte size was measured by hematoxylin/eosin staining, cluster of differentiation 68 (CD68) macrophage infiltration marker by immunohistochemistry, and adiponectin, adiponectin receptors 1 and 2, and interleukin 6 (IL6) messenger RNAs by qRT-PCR.

Results

The average EWL was 66.7 %, and CRP, triglycerides, hepatic markers, leptin levels, homeostasis model assessment, and the leptin/adiponectin ratio were lower (p < 0.05) at T1 than at T0. The expression of small adipocytes and adiponectin was increased (p < 0.05), and inflammation markers (CD68 and IL6) decreased (p < 0.05) at T1 vs. T0. At linear regression multivariate analysis, over 90 % (R2 = 0.905) of EWL (dependent variable) was explained by CD68, adiponectinemia, triglyceridemia, CRP, and total protein levels.

Conclusions

The EWL obtained 3 years after LAGB resulted in an improvement of lipid metabolism and a reduction of inflammation in NDMO patients, thereby decreasing the risk of obesity-associated diseases.

Keywords

LAGBEWLMorbid obesitySubcutaneous adipose tissueCD68

Supplementary material

11695_2014_1326_MOESM1_ESM.pdf (19 kb)
Table S1(PDF 19 kb)
11695_2014_1326_MOESM2_ESM.pdf (23 kb)
Table S2(PDF 23 kb)
11695_2014_1326_MOESM3_ESM.pdf (85 kb)
Fig. S1(PDF 85 kb)

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Laura Iaffaldano
    • 1
  • Carmela Nardelli
    • 1
    • 2
  • Vincenzo Pilone
    • 3
  • Giuseppe Labruna
    • 4
  • Andreina Alfieri
    • 5
  • Donatella Montanaro
    • 1
  • Maddalena Ferrigno
    • 1
  • Maria Restituta Zeccolella
    • 4
  • Nicola Carlomagno
    • 6
  • Andrea Renda
    • 6
  • Alfonso Baldi
    • 1
    • 7
  • Pietro Forestieri
    • 8
  • Lucia Sacchetti
    • 1
    • 2
  • Pasqualina Buono
    • 4
    • 5
  1. 1.CEINGE-Biotecnologie Avanzate S.C.a R.L.NaplesItaly
  2. 2.Dipartimento di Medicina Molecolare e Biotecnologie MedicheUniversità degli Studi di Napoli Federico IINaplesItaly
  3. 3.Dipartimento di Medicina e ChirurgiaUniversità degli Studi di SalernoFiscianoItaly
  4. 4.IRCCS Fondazione SDN–Istituto di Ricerca Diagnostica e NucleareNaplesItaly
  5. 5.Dipartimento Scienze Motorie e del BenessereUniversità degli Studi di Napoli ParthenopeNaplesItaly
  6. 6.Dipartimento di Scienze Biomediche AvanzateUniversità degli Studi di Napoli Federico IINaplesItaly
  7. 7.Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e FarmaceuticheSeconda Università degli Studi di NapoliCasertaItaly
  8. 8.Dipartimento di Medicina Clinica e ChirurgiaUniversità degli Studi di Napoli Federico IINaplesItaly