, Volume 23, Issue 4, pp 522-530
Date: 04 Dec 2012

Effects of Modified Biliopancreatic Diversion on Glucose Tolerance of GK Rats

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Abstract

Background

The purpose of this study was to investigate the hypoglycemic effect of new biliopancreatic diversion and duodenal–jejunal bypass in Goto–Kakizaki rats and observe effects of the new surgical procedure on the glucose tolerance of GK rats.

Methods

Twenty-four 10-week-old rats (SPF grade) were randomly divided into groups A, B, and C, each with eight rats. Group A underwent duodenal–jejunal bypass, group B underwent modified biliopancreatic diversion, and group C underwent a sham operation. Median rat body weight, fasting blood glucose, OGTT, and blood lipids were measured in fasting 1 week before surgery and 1, 2, 4, and 8 weeks after surgery. Changes in gastric inhibitory polypeptide, glucagon P-like peptide-1, and insulin levels were measured by ELISA 1 week before surgery and 8 weeks after surgery.

Results

Rats’ mean body weight in groups A and B decreased significantly from 368.025 ± 11.726 and 373.100 ± 9.859 g preoperatively to 345.750 ± 11.403 and 343.260 ± 12.399 g at the early postoperative stage (P < 0.05), and with statistically significant differences compared to the weight of rats in group C (P < 0.05). Comparisons between fasting blood glucose before surgery and 8 weeks after surgery revealed no significant differences between all three groups (P > 0.05). Glucose tolerance in groups A and B decreased from preoperative 21.175 ± 3.684 and 20.820 ± 1.671 mmol/L to postoperative 8.950 ± 0.580 and 10.500 ± 1.509 mmol/L, and both were better than that of group C (P < 0.001).

Conclusions

Both new biliopancreatic diversion and duodenal–jejunal bypass improve glucose tolerance of Goto–Kakizaki rats.

Supported by: the Fujian Provincial Natural Science Foundation, No. 2010J01152