Obesity Surgery

, Volume 22, Issue 11, pp 1755–1760

The Deleterious Effect of Bariatric Surgery on Cortical and Trabecular Bone Density in the Femurs of Non-obese, Type 2 Diabetic Goto-Kakizaki Rats

  • José Luis Pérez-Castrillón
  • José Antonio Riancho
  • Daniel de Luis
  • José Ramón Caeiro
  • David Guede
  • Manuel González-Sagrado
  • Marta Ruiz-Mambrilla
  • María Domingo-Andrés
  • Rosa Conde
  • David Primo-Martín
Animal Research

DOI: 10.1007/s11695-012-0732-y

Cite this article as:
Pérez-Castrillón, J.L., Riancho, J.A., de Luis, D. et al. OBES SURG (2012) 22: 1755. doi:10.1007/s11695-012-0732-y

Abstract

Background

The effects of type 2 diabetes on bone mass and microstructure are not clear. The aim of this study was to evaluate bone microstructural properties and volumetric bone mineral density (vBMD) in type 2 diabetic Goto-Kakizaki non-obese rats after gastrojejunal bypass and their relationship with hormonal parameters.

Methods

We designed an experimental study in Goto-Kakizaki rats with and without gastrojejunal bypass, performing densitometric and microstructural studies of the distal femur using X-ray computed microtomography (micro-CT). Levels of insulin, glucagon, leptin, and glucagon-like peptide-1 (GLP-1) were also determined.

Results

We observed reduced cortical (1,488.92 ± 98.2 vs. 1,727.92 ± 133.45 mg/cm3, p = 0.028) and trabecular (180.8 ± 9 vs. 261.23 ± 45.54 mg/cm3, p = 0.036) vBMD in operated rats. Bone volume fraction (BV/TV) and trabecular connectivity were reduced in operated rats, while there was a reduction in cortical thickness and an increase in rod-like trabeculae at the expense of plate-like trabeculae. Leptin was reduced (1,042 ± 549 vs. 2,447 ± 1,035 pg/ml, p = 0.05) and GLP-1 increased (1.62 ± 0.32 vs. 0.96 ± 0.1 ng/ml, p = 0.008) but only leptin showed a significant association with vBMD

Conclusions

In type 2 diabetic Goto-Kakizaki rats, gastrojejunal bypass produces a reduction in cortical and trabecular bone mineral density and a deterioration in bone quality that could be explained, in part, by the reduction in leptin levels.

Keywords

Type 2 diabetesX-ray microtomographyBone mineral densityBone microstructureLeptinGLP-1

Copyright information

© Springer Science + Business Media, LLC 2012

Authors and Affiliations

  • José Luis Pérez-Castrillón
    • 1
  • José Antonio Riancho
    • 2
  • Daniel de Luis
    • 1
  • José Ramón Caeiro
    • 3
  • David Guede
    • 4
  • Manuel González-Sagrado
    • 1
  • Marta Ruiz-Mambrilla
    • 5
  • María Domingo-Andrés
    • 1
  • Rosa Conde
    • 1
  • David Primo-Martín
    • 1
  1. 1.Institute of EndocrinologyRio Hortega University HospitalValladolidSpain
  2. 2.Marqués de Valdecilla University HospitalUniversity of Cantabria, ReticefSantanderSpain
  3. 3.Santiago de Compostela University Hospital Complex (CHUS), ReticefSantiago de CompostelaSpain
  4. 4.Trabeculae® Empresa de Base Tecnológica, S.L.OurenseSpain
  5. 5.Medical Center of Rehabilitation and LanguageValladolidSpain