Physiology Research

Obesity Surgery

, Volume 21, Issue 2, pp 231-237

First online:

Duodenal–Jejunal Bypass Surgery Does Not Increase Skeletal Muscle Insulin Signal Transduction or Glucose Disposal in Goto–Kakizaki Type 2 Diabetic Rats

  • Timothy P. GavinAffiliated withHuman Performance Laboratory, East Carolina UniversityDepartment of Exercise and Sport Science, East Carolina UniversityDepartment of Physiology, East Carolina UniversityEast Carolina Diabetes and Obesity Institute, East Carolina University Email author 
  • , Ruben C. SloanIIIAffiliated withHuman Performance Laboratory, East Carolina UniversityDepartment of Exercise and Sport Science, East Carolina University
  • , Eric Z. LukosiusAffiliated withHuman Performance Laboratory, East Carolina UniversityDepartment of Exercise and Sport Science, East Carolina University
  • , Melissa A. ReedAffiliated withHuman Performance Laboratory, East Carolina UniversityDepartment of Exercise and Sport Science, East Carolina University
  • , John R. PenderAffiliated withDepartment of Surgery, East Carolina University
  • , Van BoghossianAffiliated withDepartment of Surgery, East Carolina University
  • , Jacqueline J. CarterAffiliated withDepartment of Surgery, East Carolina University
  • , Robert D. McKernieAffiliated withHuman Performance Laboratory, East Carolina UniversityDepartment of Exercise and Sport Science, East Carolina UniversityDepartment of Physiology, East Carolina University
  • , Kushal ParikhAffiliated withHuman Performance Laboratory, East Carolina UniversityDepartment of Exercise and Sport Science, East Carolina UniversityDepartment of Physiology, East Carolina University
    • , J. William PriceAffiliated withDepartment of Physiology, East Carolina University
    • , Edward B. TapscottAffiliated withDepartment of Physiology, East Carolina University
    • , Walter J. PoriesAffiliated withDepartment of Surgery, East Carolina University
    • , G. Lynis DohmAffiliated withDepartment of Physiology, East Carolina University

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Abstract

Background

Duodenal–jejunal bypass (DJB) has been shown to reverse type 2 diabetes (T2DM) in Goto–Kakizaki (GK) rats, a rodent model of non-obese T2DM. Skeletal muscle insulin resistance is a hallmark decrement in T2DM. The aim of the current work was to investigate the effects of DJB on skeletal muscle insulin signal transduction and glucose disposal. It was hypothesized that DJB would increase skeletal muscle insulin signal transduction and glucose disposal in GK rats.

Methods

DJB was performed in GK rats. Sham operations were performed in GK and nondiabetic Wistar–Kyoto (WKY) rats. At 2 weeks post-DJB, oral glucose tolerance (OGTT) was measured. At 3 weeks post-DJB, insulin-induced signal transduction and glucose disposal were measured in skeletal muscle.

Results

In GK rats and compared to sham operation, DJB did not (1) improve fasting glucose or insulin, (2) improve OGTT, or (3) increase skeletal muscle insulin signal transduction or glucose disposal. Interestingly, skeletal muscle glucose disposal was similar between WKY-Sham, GK-Sham, and GK-DJB.

Conclusions

Bypassing of the proximal small intestine does not increase skeletal muscle glucose disposal. The lack of skeletal muscle insulin resistance in GK rats questions whether this animal model is adequate to investigate the etiology and treatments for T2DM. Additionally, bypassing of the foregut may lead to different findings in other animal models of T2DM as well as in T2DM patients.

Keywords

Type 2 diabetes Duodenal–jejunal bypass Skeletal muscle glucose disposal