Obesity Surgery

, Volume 21, Issue 3, pp 356–361

Resveratrol Upregulated SIRT1, FOXO1, and Adiponectin and Downregulated PPARγ1–3 mRNA Expression in Human Visceral Adipocytes

  • Cíntia dos Santos Costa
  • Francieli Rohden
  • Thais Ortiz Hammes
  • Rogério Margis
  • Josiane Woutheres Bortolotto
  • Alexandre Vontobel Padoin
  • Cláudio Cora Mottin
  • Regina Maria Guaragna
Basic Science Research

DOI: 10.1007/s11695-010-0251-7

Cite this article as:
Costa, C..S., Rohden, F., Hammes, T.O. et al. OBES SURG (2011) 21: 356. doi:10.1007/s11695-010-0251-7

Abstract

Background

The SIRT1 enzyme is involved in adipose tissue (AT) lipolysis. FOXO1 is a protein that plays a significant role in regulating metabolism. Adiponectin is an adipokine, secreted by the AT, which has been considered to have an antiobesity function. PPARγ is one of the key actors in adipocytes differentiation. This study was undertaken to investigate whether resveratrol can regulate SIRT1, FOXO1, adiponectin, PPARγ1–3, and PPARβ/δ in human AT.

Methods

The effects of resveratrol were analyzed in freshly isolated adipocytes prepared from visceral fat tissue samples obtained during bariatric surgery. Genes messenger ribonucleic acid (mRNA) levels were determined by qRT-PCR.

Results

Ours results show that resveratrol modulates the studied genes, increasing SIRT1 (p = 0.021), FOXO1 (p = 0.001), and adiponectin (p = 0.025) mRNA expression and decreasing PPARγ1–3 (p = 0.003) mRNA in human visceral adipocytes.

Conclusions

Resveratrol, in vitro and at low concentration, modulates genes that are related to lipid metabolism, possibly preventing metabolic disease in human visceral adipose tissue (VAT).

Keywords

AdipocytesSIRT1FOXO1AdiponectinPPARγ1–3Resveratrol

Abbreviations

AT

Adipose tissue

VAT

Visceral adipose tissue

FOXO1

Forkhead/winged helix

SIRT1

Sirtuin 1

PPAR

Peroxisome proliferator-activated receptors

qRT-PCR

Quantitative real-time polymerase chain reaction

Copyright information

© Springer Science + Business Media, LLC 2010

Authors and Affiliations

  • Cíntia dos Santos Costa
    • 1
  • Francieli Rohden
    • 1
  • Thais Ortiz Hammes
    • 1
  • Rogério Margis
    • 1
    • 2
  • Josiane Woutheres Bortolotto
    • 1
  • Alexandre Vontobel Padoin
    • 3
  • Cláudio Cora Mottin
    • 3
  • Regina Maria Guaragna
    • 1
  1. 1.Departamento de BioquímicaICBS, UFRGSPorto AlegreBrazil
  2. 2.Centro de BiotecnologiaUFRGSPorto AlegreBrazil
  3. 3.Centro de Obesidade e Síndrome MetabólicaHospital São Lucas da PUCRSPorto AlegreBrazil