Date: 13 Feb 2014

Development of a Raman chemical imaging detection method for authenticating skim milk powder

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This research developed a Raman chemical imaging method for detecting multiple adulterants in skim milk powder. Ammonium sulfate, dicyandiamide, melamine, and urea were mixed into the milk powder as chemical adulterants in the concentration range of 0.1–5.0 %. A Raman imaging system with a 785-nm laser was used to acquire hyperspectral images in the wavenumber range of 102–2,538 cm−1 for a 25 × 25 mm2 area of each mixture. A polynomial curve-fitting method was used to correct for the fluorescence background in the Raman images. An image classification method was developed based on single-band fluorescence-free images at unique Raman peaks of the adulterants. Raman chemical images were created to visualize identification and distribution of the multiple adulterant particles in the milk powder. A linear relationship was found between adulterant pixel number and adulterant concentration, demonstrating the potential of this Raman chemical imaging method for quantitative analysis of adulterants in the milk powder.