Frontiers of Medicine

, Volume 6, Issue 2, pp 173–186

Nucleic acid-based diagnostics for infectious diseases in public health affairs

Authors

    • Neuroscience Research Institute; Department of Neurobiology, School of Basic Medical Sciences; Key Laboratory for Neuroscience, Ministry of Education; Key Laboratory for Neuroscience, Ministry of HealthPeking University
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • Greg Vatcher
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • Xin Yue
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • Yan Dong
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • Mao Hua Li
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • Patrick H. K. Tam
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • Parker Y. L. Tsang
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • April K. Y. Wong
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • Michael H. K. Hui
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • Bin Yang
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
  • Hao Tang
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
    • Hai Kang Life Corporation LimitedHong Kong SAR, China
Review

DOI: 10.1007/s11684-012-0195-5

Cite this article as:
Yu, A.C., Vatcher, G., Yue, X. et al. Front. Med. (2012) 6: 173. doi:10.1007/s11684-012-0195-5

Abstract

Infectious diseases, mostly caused by bacteria and viruses but also a result of fungal and parasitic infection, have been one of the most important public health concerns throughout human history. The first step in combating these pathogens is to get a timely and accurate diagnosis at an affordable cost. Many kinds of diagnostics have been developed, such as pathogen culture, biochemical tests and serological tests, to help detect and fight against the causative agents of diseases. However, these diagnostic tests are generally unsatisfactory because they are not particularly sensitive and specific and are unable to deliver speedy results. Nucleic acid-based diagnostics, detecting pathogens through the identification of their genomic sequences, have shown promise to overcome the above limitations and become more widely adopted in clinical tests. Here we review some of the most popular nucleic acid-based diagnostics and focus on their adaptability and applicability to routine clinical usage. We also compare and contrast the characteristics of different types of nucleic acid-based diagnostics.

Keywords

nucleic acid-based diagnosticsinfectious diseasePCRNASBALAMPmicroarrayLOACpublic health affairs

Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2012