Carbon stock estimation was conducted in tree species of Sem Mukhem sacred forest in district Tehri of Garhwal Himalaya, Uttarakhand, India. This forest is dedicated to Nagraj Devta and is dominated by tree species, including Quercus floribunda, Quercus semecarpifolia and Rhododendron arboreum. The highest values of below ground biomass density, total biomass density and total carbon density were (34.81±1.68) Mg·ha−1, (168.26±9.04) Mg·ha−1 and (84.13±4.18) Mg·ha−1 for Pinus wallichiana. Overall values of total biomass density and total carbon density calculated were 1549.704 Mg·ha−1 and 774.77 Mg·ha−1 respectively. Total value of growing stock volume density for all species was 732.56 m3·ha−1 and ranged from (144.97±11.98) m3·ha−1 for Pinus wallichiana to (7.78±1.78) m3·ha−1 for Benthamidia capitata.