Journal of Forestry Research

, Volume 20, Issue 3, pp 195–198

Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica

  • Pascal Eusemann
  • Steffen Fehrenz
  • Martin Schnittler
Research Paper

DOI: 10.1007/s11676-009-0038-7

Cite this article as:
Eusemann, P., Fehrenz, S. & Schnittler, M. Journal of Forestry Research (2009) 20: 195. doi:10.1007/s11676-009-0038-7

Abstract

Eighteen microsatellite primer pairs previously developed at Oak Ridge National Laboratory for Populus tremuloides Michx. and Populus trichocarpa Torr. & Gray were screened for amplification in Euphrates poplar, Populus euphratica Oliv. Thirteen loci were found to express polymorphisms ranging from two to 17 alleles. The eight most variable loci were selected to set up and optimize two multiplex polymerase chain reaction (PCR) assays. Three populations containing altogether 436 trees were used to characterize the selected loci and ascertain their applicability for parentage analysis and genotyping studies. Through cross-checking of clonal identity against sex of the genotyped trees we estimated the maximum error rate for merging genotypes to be less than 0.045.

Keywords

clone identificationEuphrates poplargenetic fingerprintparentage analysispopulation structureSSR primers

Copyright information

© Northeast Forestry University and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Pascal Eusemann
    • 1
  • Steffen Fehrenz
    • 1
  • Martin Schnittler
    • 1
  1. 1.Department of Botany and Landscape Ecology, EMAU GreifswaldGreifswaldGermany